en ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Background:</span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Two newly identified proteins, EspB and EspC are involved in the pathogenesis of <i>Mycobacterium tuberculosis</i>. The objective of the present study was to evaluate the immunogenicity of recombinant EspC, EspB,<i> </i>and EspC/EspB fusion<i> </i>proteins in mice.</span></span></span><br> <span lang="IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span><span lang="IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span><span lang="IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Methods:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">BALB/c mice were immunized subcutaneously with recombinant EspC, EspB, and fusion EspC/EspB proteins, three times with along with Quil-A as an adjuvant. The cellular and humoral immune responses were evaluated by quantifying IFN-</span></span><span style="font-size:12.0pt"><span style="font-family:Symbol">g</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, IL-4, IgG, IgG1, and IgG2a antibodies against the antigens.</span></span><br> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span></span></span></span></span><br> <b><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Results:</span></span></span></span></i></b> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">The results showed that the mice immunized with recombinant EspC, EspB, and EspC/EspB proteins did not produce IL-4, whereas IFN-</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:Symbol">g</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> was secreted in response to all three proteins. <span style="color:black">EspC/EspB group produced significant amounts of IFN-</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:Symbol"><span style="color:black">g</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> in response to stimulation with all the three recombinant proteins (P<0.001). In mice immunized with EspC, high levels of IFN-</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:Symbol"><span style="color:black">g</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were detected in response to EspC/EspB, and EspC (P<0.0001); while mice immunized with EspB produced lower levels of IFN-</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:Symbol"><span style="color:black"> g</span></span></span></span> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">in response to EspC/EspB, and EspB (P<0.05).</span></span></span></span><span style="font-size:14.0pt"><span style="line-height:107%"></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">All the three recombinant proteins induced Th1-type immune responses in mice against EspB and EspC; however, EspC/EspB protein is more desirable due to the presence of epitopes from both EspC and EspB proteins and the production of immune responses against both.</span></span></span></span></span></span></span></div> EspB, EspC, ESX-1, Mycobacterium tuberculosis. 590 598 http://rbmb.net/browse.php?a_code=A-10-139-4&slc_lang=en&sid=1 Omid Salemi 100319475328460016798 100319475328460016798 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Zahra Noormohammadi 100319475328460016799 100319475328460016799 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Fariborz Bahrami 100319475328460016800 100319475328460016800 No Department of Immunology, Pasteur Institute of Iran, 69 Pasteur Ave, Tehran 13169-43551, Iran. Seyed Davar Siadat 100319475328460016801 100319475328460016801 No Department of Mycobacteriology and pulmonary research, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran 13169-43551, Iran. Soheila Ajdary ajdsoh@pasteur.ac.ir. 100319475328460016802 100319475328460016802 Yes Department of Immunology, Pasteur Institute of Iran, 69 Pasteur Ave, Tehran 13169-43551, Iran.