Reports of Biochemistry and Molecular Biology

en Role of Long non Coding RNAs, NEAT1 and Lnc-DC Expression in Pediatric Immune Thrombocytopenic Purpura زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Pediatric immune thrombocytopenic purpura (ITP) is an autoimmune disease; whose etiology is unknown. lncRNAs are regulators of numerous actions, which participate in the development of autoimmune diseases. We evaluated the expression ofNEAT1 and Lnc-RNA in dendritic cell (Lnc-DC) in pediatric ITP. Methods: Sixty ITP patients and 60 healthy subjects were enrolled in the present study; Real-time PCR was performed to assess the expression levels of NEAT1 and Lnc-DC in sera of children with ITP as well as healthy children. Results: Both lncRNAs, NEAT1 and Lnc-DC were significantly upregulated in ITP patients in comparison to controls (p <0.0001 and P= 0.001 respectively). Furthermore, significant upregulation of the expression levels of NEAT1 and Lnc-DC were observed in the non-chronic compared with chronic ITP patients. Also, there was significant negative correlation between each of NEAT1 and Lnc-DC and platelet counts before treatment (r= -0.38; P= 0.003 and r= -0.461; P< 0.0001, respectively). Conclusions: serum lncRNAs, NEAT1 and Lnc-DC could be used as potential biomarkers in differentiating childhood ITP patients and healthy controls in addition to differentiating non-chronic from chronic ITP which may provide a theoretical basis for the mechanism and treatment of immune thrombocytopenia.</div> Lnc-DC, NEAT1, Pediatric, ITP. 635 643 http://rbmb.net/browse.php?a_code=A-10-1170-1&slc_lang=en&sid=1 Soha Mohamed Hamdy 100319475328460016821 100319475328460016821 No Departments of Chemistry, Faculty of Science, Fayoum University, Fayoum, Egypt. Marwa Saad Ali 100319475328460016822 100319475328460016822 No Departments of Chemistry, Faculty of Science, Fayoum University, Fayoum, Egypt. Rehab Galal Abd El-Hmid 100319475328460016823 100319475328460016823 No Departments of Pediatrics, Faculty of Medicine, Fayoum University, Egypt. Noha Khalifa Abdelghaffar nka00@fayoum.edu.eg 100319475328460016824 100319475328460016824 No Departments of Clinical pathology, Faculty of Medicine, Fayoum University, Egypt. Omayma Owees Abdelaleem 100319475328460016825 100319475328460016825 Yes Departments of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Egypt.