Reports of Biochemistry and Molecular Biology

en Does Gold-Silver Core-Shell Nanostructure with Alginate Coating Induce Apoptosis in Human Lymphoblastic Tumoral (Jurkat) Cell Line? زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3). Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia. Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3). Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.</div> Apoptosis, Cell viability, Nanostructures, T-cell acute lymphoblastic leukemia. 233 240 http://rbmb.net/browse.php?a_code=A-10-1231-1&slc_lang=en&sid=1 Jamileh Sadat Mirsanei 100319475328460017914 100319475328460017914 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mahsa Nazari 100319475328460017915 100319475328460017915 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Ronak Shabani 100319475328460017916 100319475328460017916 No Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran. Azam Govahi 100319475328460017917 100319475328460017917 No Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran. Sahar Eghbali 100319475328460017918 100319475328460017918 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Marziyeh Ajdary 100319475328460017919 100319475328460017919 No Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran. Rana Mehdizadeh 100319475328460017920 100319475328460017920 No School of Dentistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Atieh Sadat Mousavi 100319475328460017921 100319475328460017921 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mehdi Mehdizadeh Mehdizadeh.m@iums.ac.ir 100319475328460017922 100319475328460017922 Yes Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.