en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Background:</span></span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">The incidence of prostate cancer (PC) exhibits geographical heterogeneity. However, the metabolic mechanisms underlying this geographic heterogeneity remain unclear. This study aimed to reveal the metabolic mechanism of the geographic heterogeneity in the incidence of PC.</span></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">This study aimed to investigate the anti-cancer effects of different gum extracts on metabolic changes and their impact on gene expression in HT-29 cell.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Methods:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Transcriptomic data from public databases were obtained and analyzed to screen geographic-differentially expressed genes and metabolic pathways. Associations between these differentially expressed genes and the incidence of PC were determined to identify genes that were highly associated with PC incidence. A co-expression network analysis was performed to identify geographic-specific regulatory pathways.</span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Results:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">A total of 175 differentially expressed genes were identified in four countries and were associated with the regulation of DNA replication and the metabolism of pyrimidine, nucleotides, purines, and galactose. Additionally, the expression of the genes <i>CLVS2</i>, <i>SCGB1A1</i>, <i>KCNK3</i>, <i>HHIPL2</i>, <i>MMP26,</i> <i>KCNJ15</i>, and <i>PNMT</i> was highly correlated with the incidence of PC. Geographic-specific differentially expressed genes in low-incidence areas were highly correlated with <i>KCNJ15</i>, <i>MMP26</i>, <i>KCNK3</i>, and <i>SCCB1A1</i>, which play a major role in ion channel-related functions.</span></span></span><br> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">This study suggests that geographic heterogeneity in PC incidence is associated with the expression levels of genes associated with amino acid metabolism, lipid metabolism, and ion channels.</span></span></span></span></span></span></span></span></span></span><br> &nbsp;</div> Differentially expressed genes, Geographical heterogeneity, Metabolic pathway, Prostate cancer. 92 101 http://rbmb.net/browse.php?a_code=A-10-1254-1&slc_lang=en&sid=1 Yi Zheng 100319475328460017831 100319475328460017831 No Center for Medical Experiments (CME), University of Chinese Academy Sciences-Shenzhen Hospital, Shenzhen, China. Yang Wang 100319475328460017832 100319475328460017832 No The Department of Urology of Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China. Haitian He 100319475328460017833 100319475328460017833 No The Department of Urology of Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China. Zhuping Zou 100319475328460017834 100319475328460017834 No The Operating Room of Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China. Huiling Lu 100319475328460017835 100319475328460017835 No The Operating Room of Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China. Jinlong Li kebingwang2005@sohu.com 100319475328460017836 100319475328460017836 No TB Healthcare Co., Ltd., Foshan, China. Jun Cai 100319475328460017837 100319475328460017837 No TB Healthcare Co., Ltd., Foshan, China. Kebing Wang kebingwang2005@163.com 100319475328460017838 100319475328460017838 Yes The Department of Urology of Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China & The Department of Urology of Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, China.