Reports of Biochemistry and Molecular Biology

en Streptococcus Mutans Serotype Analysis from Dental Plaque of Caries Patients in Banda Aceh Based on the GTF Gene میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;">Background: Dental caries is an oral disease that is widely suffered by the population of Aceh caused by Streptococcus mutans. S. mutans serotypes c and d are widely isolated in the human oral cavity. This research was focused on detecting the presence and variability of S. mutans in supragingival dental plaque of caries teenager and young adults’ patients. Methods: Subjects involved in this study were patients who treated at the Rumah Sakit Gigi dan Mulut of Dentistry Faculty of Universitas Syiah Kuala. The approach used in this research was molecular microbiology technique. To determine the presence of S. mutans, supragingival plaque from caries patients was cultivated in TYS20B. The culture findings were utilized to detect the presence of bacteria using PCR. The primers utilized in the PCR were S. mutans specific primers, GTFB (517 bp) for S. mutans serotype c and GTFI (712 bp) for S. mutans serotype d. Results: Culture results on TYS20B media showed the growth of S. mutans colonies isolated from the supragingival plaque of research subjects. PCR results also revealed the presence of S. mutans in the supragingival plaques of caries patients, with the variability of S. mutans discovered to be a serotype c and a serotype d. Conclusions: Based on the findings of this study, it can be concluded that S. mutans can be found in the supragingival plaques of caries patients with the serotypes c and d variability.</div> Cariogenic, Dental Caries, Streptococcus mutans, Supragingival Plaque, TYS20B. 205 210 http://rbmb.net/browse.php?a_code=A-10-1270-1&slc_lang=en&sid=1 Santi Chismirina 100319475328460017896 100319475328460017896 No Graduate School of Department of Mathematics and Science Application, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia & Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia. Suzanna Sungkar 100319475328460017897 100319475328460017897 No Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia. Muhammad Adlim 100319475328460017898 100319475328460017898 No Department of Chemistry, FKIP, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia. Darmawi Darmawi darmawi@usk.ac.id 100319475328460017899 100319475328460017899 Yes Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia.