Reports of Biochemistry and Molecular Biology

en Vitamin D and miRNA-155 in Behçet’s Disease: Possible Association with the Disease and Disease Activity ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;">Background: Vitamin D (vit D) controls inflammation and immunity. In Behçet’s disease (BD), microRNA-155 is recognized as a significant immune response regulator. We aimed to investigate the role of vit D on immunomodulation and downregulation of inflammatory pathways associated with BD and detect the role of miRNA-155 in BD. Methods: miRNA-155 expression by Real Time -Polymerase Chain Reaction (RT-PCR), and vit D, nuclear factor Kappa-light-chain-enhancer of activated B cells (NF-κB), and Tumor necrosis fact of TNF-α) expression by Enzyme Linked Immunosorbent Assay (ELISA) were assessed. Results: BD patients had a significantly higher relative expression of microRNA-155 (P< 0.001), it was significantly related to vascular manifestations (P< 0.001). Vit D relative expression was significantly low in BD (P< 0.001). There was a significant rise in miRNA-155 in the active group compared to the inactive group (P< 0.001). A significant decrease in vit D levels (IU) was found in inactive and active individuals suffering from BD when compared to controls (P< 0.001). A significant rise was found in vit D levels in inactive BD cases (P< 0.001). A significant positive correlations were found between miRNA-155, NF-κB, TNF-α, and negative correlations with vit D relative expression in BD patients. Conclusions: miRNA-155 relative expression is higher in BD is significantly related to vascular manifestations. It may have a relationship to disease activity. Vitamin D relative expression is significantly low in BD patients, which can significantly influence immunomodulatory BD therapy. Vitamin D deficiency linked to active BD.</div> miRNA-155, Reverse Transcriptase Polymerase Chain Reaction Behçet’s disease, Vitamin D. 251 258 http://rbmb.net/browse.php?a_code=A-10-1284-1&slc_lang=en&sid=1 Dina Mohamed Mekawy 100319475328460017930 100319475328460017930 No Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University, Egypt & Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University, Egypt. Mervat Eissa mervateissa@kasralainy.edu.eg 100319475328460017931 100319475328460017931 Yes Rheumatology Department, Faculty of Medicine, Cairo University, Egypt. Noha Adly Sadik 100319475328460017932 100319475328460017932 No Internal medicine Faculty of Medicine, Cairo University, Egypt. Rana Mohamed Abd-Elrahman 100319475328460017933 100319475328460017933 No Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University, Egypt. Ahmed Fawzy 100319475328460017934 100319475328460017934 No Medical Physiology, Faculty of Medicine, Cairo University, Egypt. Marwa Fathy Amer 100319475328460017935 100319475328460017935 No Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University, Egypt.