Reports of Biochemistry and Molecular Biology

en Interleukin-10 Gene Polymorphisms Modulate the Risk of Infertility in Chlamydia trachomatis Positive Kurdish Women in Erbil Province ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;">Background: There is evident inter-individual variability in women's responses to Chlamydial infections and reproductive tract problems. Women's genetic variations within the Interleukin-10 (IL-10) gene have been linked to variances in response to Chlamydia trachomatis infection. This study was aimed to demonstrate the profound association of IL-10 with infertility and demonstrate the role of IL-10 (-592 C/A rs1800872) and (-1082 A>G rs1800896) single nucleotide polymorphism (SNPs) gene in the susceptibility and severity of a C. trachomatis infection. Method: In this evaluation study, serum IL-10 concentration was measured in 134 women diagnosed with infertility and 50 healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs1800872 and rs1800896 SNPs genes. Result: Both female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital C. trachomatis by PCR was 46.2% in infertile women. The serum concentration of IL10 was lower in infertile women than healthy participants and higher in infertile C. trachomatis-positive women compared to infertile C. trachomatis-negative in all groups except endometriosis (Endo) infertility. In rs1800872, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs1800896, the AG genotype and G allele show a greater risk for infertility. Conclusions: Our results confirmed that rs1800872 and rs1800896 gene polymorphisms were associated with an increased risk of C. trachomatis infection.</div> Chlamydia trachomatis, Female infertility, IL-10, SNP 550 565 http://rbmb.net/browse.php?a_code=A-10-1340-1&slc_lang=en&sid=1 Lawin Ahmed Omar Lawin.omar@su.edu.krd 100319475328460020218 100319475328460020218 Yes Department of Biology, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq Suhaila Nafee Darogha Nafee Darogha 100319475328460020219 100319475328460020219 No Department of Biology, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq