Reports of Biochemistry and Molecular Biology

en Serum miR-23 and miR-150 Profiles as Biomarkers for Predicting Recurrence following Surgical Intervention in Colorectal Cancer Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div class="WordSection1" style="text-align: justify;">Background: MicroRNAs (miRNAs) play pivotal roles in post-transcriptional regulation of gene expression and have emerged as crucial regulators in cancer development, progression, and metastasis. This study aimed to assess the expression profiles of miR-23, miR-223, miR-1246, and miR-150 in serum samples obtained from colorectal cancer (CRC) patients before and three months after surgery, in comparison to a healthy control group, to explore their biomarker potential. Methods: A total of 50 blood samples were collected from patients with CRC (pre- and post-surgery), along with 50 samples from healthy controls. The relative expression levels of miR-23, miR-223, miR-1246, and miR-150 in the serum were quantified using quantitative real-time PCR. Results: Our findings revealed upregulated expression levels of miR-23, miR-1246, and miR-223, while miR-150 exhibited significant downregulation in the serum of CRC subjects compared to healthy controls. Receiver operating characteristic (ROC) analysis indicated that miR-23 and miR-150 could distinguish CRC cases from controls with relatively high accuracy. Moreover, three months post-surgery, miR-23, miR-1246, and miR-223 serum levels were downregulated, and miR-150 was significantly upregulated. However, no significant correlations were observed between serum levels of the studied genes and the clinical features of our patients. Conclusions: The serum levels of miR-23 and miR-150 hold promise as potential biomarkers for the diagnosis and prognosis of CRC.</div> Biomarker, Colorectal cancer, micro-RNAs, Tumorigenesis. 540 549 http://rbmb.net/browse.php?a_code=A-10-1435-1&slc_lang=en&sid=1 Saeid Mahmoudivar 100319475328460018316 100319475328460018316 No Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Habib Zarredar 100319475328460018317 100319475328460018317 No Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Milad Asadi 100319475328460018318 100319475328460018318 No Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey. Venus Zafari 100319475328460018319 100319475328460018319 No Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey. Shahriyar Hashemzadeh 100319475328460018320 100319475328460018320 No Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Rojin Farzaneh 100319475328460018321 100319475328460018321 No Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Touraj Asvadi Kermani Tooraj_asvadi2005@yahoo.com 100319475328460018322 100319475328460018322 Yes Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.