Reports of Biochemistry and Molecular Biology

en Expression Levels of miR -124a, miR-545-3p and BDNF in the Peripheral Blood Mononuclear Cells Are Associated with the Severity of Autism زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div class="WordSection1"> <div style="text-align: justify;">Background: People with autism frequently exhibit poor social skills, communication difficulties, and repetitive and stereotyped behaviors. MicroRNAs (miRNAs) are potential and promised targets in developing of new treatment strategies for autism.This study aimed to assess the relative expression of miR-124a, miR-34a-3p, miR-545-3p, miR-153, and BDNF in the blood samples of autistic children. Methods: The children autism rating scale (CARS) was used to determine the severity of autism and to confirm the diagnosis. Blood samples were obtained from 50 patients and 40 age-/sex-matched healthy controls. Expressions of miR-545-3p, miR-34a-3p, miR-124a, and BDNF were evaluated using qRT-PCR. Pearson's correlation coefficient and regression analysis were used to check correlations between relative expressions of the miRNAs and BDNF. Biomarker potencies were assessed by ROC curve analysis. Results: qRT-PCR analysis showed that the relative expressions of miR-545-3p, miR-34a-3p, miR-124a, and BDNF were significantly higher in the patients' group than the healthy controls. However, the relative expression of miR-153 was significantly lower in the case group than the control group. The relative expression of miR-124a was positively correlated with those of miR-545-3p and BDNF among the patients group. Also, the relative expressions of miR-545-3p and BDNF were positively correlated with each other. The ROC curve data also indicated that miR-124a, miR-34a-3p, miR-545-3p, miR-153, and BDNF could be possible diagnostic biomarker for CARS diagnosis (AUC=0.8328, AUC=0.8354, AUC=0.6727, AUC=0.8518 and AUC=0.8214, respectively). Conclusions: Deregulation of miR-124a, miR-454-3p and BDNF might be considered as potential biomarkers for severity of autism.</div> <div align="center" style="text-align:center"> <hr align="center" noshade="noshade" size="2" style="color:#0f243e" width="100%" ></div> </div> <div style="text-align: justify;"><div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div></div> Autistic children, Autism, Biomarker, Brain-derived neurotrophic factor, Gene expression, miRNA. 1 12 http://rbmb.net/browse.php?a_code=A-10-1427-3&slc_lang=en&sid=1 Safa Jasim Tuama Ali 100319475328460020350 100319475328460020350 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Mohammad Khalaj-Kondori khalaj@tabrizu.ac.ir 100319475328460020351 100319475328460020351 Yes Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Mohammad Ali Hoseinpour Feizi 100319475328460020352 100319475328460020352 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Mehdi Haghi 100319475328460020353 100319475328460020353 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.