en تخصصي Special مقالات اصلی Original Article <p style="text-align: justify;"><strong><em>Background:</em></strong> The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia.</p> <p style="text-align: justify;"><strong><em>Methods:</em></strong> In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary<strong> (</strong>CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-h<em>IL-7 </em>recombinant by electroporation. Expression of rhIL-7 in CHO-K1 cells was confirmed by enzyme-linked immunosorbent assay (ELISA) and dot and western blots.</p> <p style="text-align: justify;"><strong><em>Results:</em></strong> On western blots of transformed cells, a single 25 kDa band was observed, consistent with the expected molecular weight of glycosylated hIL-7. No significant expression difference was observed between cells transfected with circular or linear plasmids.</p> <p style="text-align: justify;"><strong><em>Conclusions:</em></strong> We established a stable CHO-K1 cell line expressing rhIL-7, which we consider to be a promising system for the production of rhIL-7 as a biopharmaceutical.</p> CHO cells, Interleukin-7, Post-translational modifications, Stable transfection. 66 73 http://rbmb.net/browse.php?a_code=A-10-1-85&slc_lang=en&sid=1 Fatemeh Sadat Toghraie 10031947532846002237 10031947532846002237 No Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran. Seyedeh Masoumeh Sharifzadeh 10031947532846002238 10031947532846002238 No Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Amin Ramezani 10031947532846002239 10031947532846002239 No Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. Elham Mahmoudi Maymand 10031947532846002240 10031947532846002240 No Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran. Mahsa Yazdanpanah-Samani 10031947532846002241 10031947532846002241 No Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran. Abbas Ghaderi ghaderia@sums.ac.ir 10031947532846002242 10031947532846002242 Yes Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.