en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.4pt">Background:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Investigating the role of&nbsp;glutathione S-transferase pi 1&nbsp;(GSTP1)&nbsp;in&nbsp;breast cancer development and exploring&nbsp;genetic variations&nbsp;in GSTP1 that may contribute to susceptibility to the disease.</span></span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Methods:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt"> Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction.&nbsp;PCR&nbsp;and&nbsp;bioinformatics analysis were used to examine the GSTP1 gene sequences.</span></span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Results:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt"> Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions,&nbsp;with&nbsp;homozygous CC&nbsp;and&nbsp;heterozygous CA genotypes. Patient samples with the CA genotype&nbsp;were submitted to NCBI under&nbsp;accession numbers OL957029-OL957036, while those with the CC genotype&nbsp;were submitted&nbsp;under&nbsp;accession numbers OL957027-OL957034. Additionally,&nbsp;sequence analysis of&nbsp;the&nbsp;rs757152293 SNP in healthy individuals&nbsp;was&nbsp;submitted to NCBI under&nbsp;accession numbers OL957037-OL957041.</span></span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Conclusion:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt"> This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.</span></span></span></span></span></span></span></span></span></span></div> Breast Cancer, Glutathione S-Transferase Pi 1, Iraq, Polymorphism. 516 524 http://rbmb.net/browse.php?a_code=A-10-1675-1&slc_lang=en&sid=1 Hasan Alhayali 100319475328460021648 100319475328460021648 No Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Iran. Zohreh Hojati z.hojati@sci.ui.ac.ir. 100319475328460021649 100319475328460021649 Yes Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Iran.