en ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> The quality of extracts used in the skin prick test directly influences the interpretation of the test. Accordingly, the outcomes and effectiveness of immunotherapy for the management of IgE-mediated allergies depend on the quality of the extracts used. Excipients, which are pharmacologically inert ingredients, are intentionally added to the active ingredients. The aim of this study was to address optimum excipients for stability <em>Platanus (P.) orientalis </em>extract.<br> <br> <strong><em>Methods:</em></strong> In this study the excipients examined were l-lysine (20 mM), l-cysteine (20 mM), albumin (0.5%), sorbitol (2%), sucrose (750 mM), trehalose (20 mM), D-mannitol (2% w/v), urea (100 mM) and Tween-20 (0.1%). Their effects on <em>P. orientalis</em> extract stability were analyzed using an inhibition enzyme linked immune assay at 37 ᵒC.<br> <br> <strong><em>Results:</em></strong> A mixture of lysine (20 mM), trehalose (20 mM), and D-mannitol (2% w/v) conferred the greatest stability on the <em>P. orientalis</em> extract.<br> <br> <strong><em>Conclusions:</em></strong> The <em>P. orientalis</em> extract stability was increased by a mixture of lysine (20 mM), trehalose (20 mM), and D-mannitol.<br> &nbsp;</div> Lysine, Mannitol, Platanus orientalis, Skin prick test, Trehalose. 189 195 http://rbmb.net/browse.php?a_code=A-10-114-1&slc_lang=en&sid=1 Hasan Mansouritorghabeh mansouritorghabeh@mums.ac.ir 100319475328460017075 100319475328460017075 No Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Mojtaba Sankian sankianm@mums.ac.ir 100319475328460017076 100319475328460017076 No Immunology Research Center, Buali Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Abdol-Reza Varasteh varastehAR@mums.ac.ir 100319475328460017077 100319475328460017077 No Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Malihe Moghadam Moghadamm1@mums.ac.ir 100319475328460017078 100319475328460017078 No Immunology Research Center, Buali Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Bahman Khameneh khamenehbagheriB@mums.ac.ir 100319475328460017079 100319475328460017079 No Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Reza Farid-Hosseini rfaridh@yahoo.com 100319475328460017080 100319475328460017080 No Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Farahzad Jabbari-Azad Jabbarif@mums.ac.ir 100319475328460017081 100319475328460017081 Yes Allergy Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.