en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Diabetes mellitus and metabolic disorders are a major burden on the healthcare system. Irisin is a novel myokine reported to have beneficial effects on glucose and lipid metabolism. Vitamin D deficiency has been implicated in the development of diabetes and hold a critical role in diabetes-related complications. In the present study, we examined the efficacy of vitamin D supplementation on serum irisin levels, skeletal muscle irisin levels, and the expression of the irisin precursor, FNDC5 (fibronectin-type III domain-containing 5) in type I diabetes mellitus rats.<br> <br> <strong><em>Methods:</em></strong> Thirty-six adult male Sprague-Dawley rats (150 &ndash; 250 g) were randomly divided into four groups: group I: healthy control rats with no treatment (n=8), group II: healthy control rats receiving sesame oil as a placebo (n=8), group III: diabetic rats receiving sesame oil as placebo (n=10), group IV: diabetic rats treated with 4300 IU/kg/week vitamin D (n=10). Diabetes was induced by intraperitoneal (IP) injection of streptozotocin. At the end of the vitamin D intervention blood and triceps muscle samples were collected. RNA was extracted from muscle and real-time PCR was performed to examine FNDC5 gene expression.<br> <br> <strong><em>Results:</em></strong> Our study showed that the administration of vitamin D (4300 IU/kg/week) in a streptozotocin-diabetic rat model resulted in increased serum vitamin D levels, FNDC5 gene expression and muscle irisin levels. However, the levels of serum irisin were not significantly changed by the administration of vitamin D.<br> <br> <strong><em>Conclusions:</em></strong> In conclusion, we show that vitamin D supplementation enhances serum vitamin D levels, FDNC5 gene expression and muscle irisin levels in the streptozotocin-diabetic rat model. Our study highlights the potential therapeutic effect of vitamin D supplementation for diabetes mellitus.</div> Diabetes, FNDC5, Irisin, Vitamin D. 236 243 http://rbmb.net/browse.php?a_code=A-10-83-4&slc_lang=en&sid=1 Hoda Nadimi nadimi.h@gmail.com 100319475328460017331 100319475328460017331 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Abolghassem Djazayery djazayery@gmail.com 100319475328460017332 100319475328460017332 No Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hassan Javanbakht nhjavan2001@gmail.com 100319475328460017333 100319475328460017333 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Ahmadreza Dehpour dehpour.a@gmail.com 100319475328460017334 100319475328460017334 No Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. Ehsan Ghaedi ghaedi98@gmail.com 100319475328460017335 100319475328460017335 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Hoda Derakhshanian derakhshanian@yahoo.com 100319475328460017336 100319475328460017336 No Department of Biochemistry, Nutrition and Genetics, Medical School, Alborz University of Medical Sciences, Karaj, Iran. & Dietary supplements and probiotics Research Center, Alborz University of Medical Sciences, Karaj, Iran. Hamed Mohammadi mohamadihd@gmail.com 100319475328460017337 100319475328460017337 No Student Research Committee, Department of Clinical Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran. Mahnaz Zarei zareiii@yahoo.com 100319475328460017338 100319475328460017338 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mahmoud Djalali mjalali87@yahoo.com 100319475328460017339 100319475328460017339 Yes Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.