en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> In recent years, prostate cancer prevails as one of the lead cancers affecting men. Currently, prostate cancer research involves the phytochemical study of plants with anti-tumour effects. This study compares the anti-tumour effects of three plant species indigenous to Iran and their interaction with cluster of differentiation (CD)-82 protein, a therapeutic target found in prostate cancer cells.<br> <br> <strong><em>Methods:</em></strong> The extracts of <em>Hypericum perforatum</em>, <em>Achillea millefolium</em>, and <em>Aloe vera</em> were prepared and their toxicological, cellular and gene expression responses were evaluated in PC-3 human prostate cancer cells and normal human chondrocyte cell line C28/I2. They were exposed to different concentrations of the plants (10 mg/mL, 5 mg/mL, 1 mg/mL, 100 &micro;g/mL, 10 &micro;g/mL, and 1 &micro;g/mL) at three exposure time points (24, 48, 72 hours) to determine cancer cell cytotoxicity and gene expression profiles.<br> <br> <strong><em>Results</em></strong><strong><em>:</em></strong> Half-maximal inhibitory concentration (IC50) in PC-3 cells ranged from 0.6 to 8.5 mg/mL for <em>H. perforatum </em>extract, from 0.4 to 7.5 mg/mL for <em>A. Millefolium</em> extract, and from 0.2 to 8.0 mg/mL for <em>A. vera </em>extract in a time-dependent manner. <em>A. vera </em>extract caused the highest cell death levels in PC-3 cells (94%) and C28/I2 cells (57%) after 48 hours. A 1.97-, 3.00-, and 3.48-fold increase in relative gene expression of CD82 was observed for <em>H. perforatum</em>, <em>A. millefolium</em>, and <em>A. vera </em>extracts, respectively<br> <br> <strong><em>Conclusions:</em></strong> <em>A. vera</em> and <em>A. millefolium</em> extracts are a selective inhibitor of prostate cancer cells and a potent activator of CD82 expression.<br> &nbsp;</div> CD82, Gene expression, Herbal medicine, Prostate cancer. 260 268 http://rbmb.net/browse.php?a_code=A-10-320-1&slc_lang=en&sid=1 Motahare-Sadat Hosseini hosseini181@gmail.com 100319475328460017354 100319475328460017354 No Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran. Fatemehsadat Hosseini hosseinif6838@gmail.com 100319475328460017355 100319475328460017355 No Faculty of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Abdolreza Ahmadi aa_13651363@yahoo.com 100319475328460017356 100319475328460017356 No Faculty of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Masoud Mozafari mozafari.masoud@gmail.com 100319475328460017357 100319475328460017357 No Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Issa Amjadi gc0645@wayne.edu 100319475328460017358 100319475328460017358 Yes Department of Biomedical Engineering, Wayne State University, Detroit, MI, United States.