en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Selenium is a mineral that showed both pro- and anti-oxidant activities in various disease models. In this study, we evaluated the anti-tumor effect of selenium against 1,2-dimethylhydrazine (DMH)-induced colorectal cancer in BALB/C mice and its effect on apoptosis and angiogenesis.<br> <br> <strong><em>Methods:</em></strong> Colorectal cancer was induced by subcutaneous injection of DMH (20 mg/kg body weight) in BALB/C mice once weekly for 20 weeks. Selenium (200 mg/L) was given to DMH plus selenium-treated group in the drinking water for the next 3 months.<br> <br> <strong><em>Results:</em></strong> The DMH plus selenium-treated group exhibited significantly lower expression of cloned caudal-type homebox gene -2 (CDX-2) and vascular endothelial growth factor (VEGF) but higher caspase-3 expression level at p<0.001 compared to the DMH-treated group. Moreover, a decrease in the reduced glutathione content and glutathione peroxidase activity but an increase in the malondialdehyde content were observed at p<0.001. Both macroscopic and microscopic examination of the colorectal tissues confirmed the results.<br> <br> <strong><em>Conclusions:</em></strong> The anti-tumor effect of selenium against an induced colorectal cancer in mice is attributed to its pro-oxidant, anti-angiogenic and apoptotic effects.</div> Angiogenesis, Apoptosis, Cancer, Mineral, Oxidative Stress. 216 226 http://rbmb.net/browse.php?a_code=A-10-345-1&slc_lang=en&sid=1 Mohammed Saeed Ali mohamed.ali@pharm.bsu.edu.eg 100319475328460017326 100319475328460017326 No Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt. Rasha Mohamed Hussein rasha.hussein@pharm.bsu.edu.eg 100319475328460017327 100319475328460017327 Yes Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt & Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Mutah University, 61710, Al-Karak, Jordan. Mohamed Ahmed Kandeil mohamed.kandeal@vet.bsu.edu.eg 100319475328460017328 100319475328460017328 No Department of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.