en بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Obesity, often associated with insulin resistance and type 2 diabetes, is a metabolic disease that can result in dyslipidemia and hyperglycemia. Many reports describe the hypoglycemic and hypolipidemic properties of the&nbsp;<em>Phoenix dactylifera</em>&nbsp;L. seed extract in STZ-induced diabetic rat models, however, its anti-diabetic effects in other diabetic models are less characterized in the literature. This study set out to determine the possible effects of the&nbsp;<em>Phoenix dactylifera&nbsp;</em>L. seed extract on adipogenesis and glucose homeostasis.&nbsp;<br> <br> <strong><em>Methods:</em></strong> 3T3-L1 cells were cultured in adipocyte differentiation media with or without varying doses of&nbsp;<em>Phoenix dactylifera&nbsp;</em>L. extract (0.312-1 mg/ml). Assays were performed on days 5, 8, and 12 after induced differentiation.&nbsp;<br> <br> <strong><em>Results</em></strong><strong><em>:</em></strong> Our results demonstrate that the triglyceride content in treated groups was significantly lower compared to controls. Further, treating 3T3-L1 cells with&nbsp;<em>Phoenix dactylifera L.&nbsp;</em>seed extract reduced adipogenesis through the downregulation of PPAR-&gamma; and CEBP-&alpha;, and adipocyte-specific genes involved in fatty acid metabolism including ap2, ACACA, and FAS.&nbsp;<br> <br> <strong><em>Conclusions:</em></strong> <em>Phoenix dactylifera L.&nbsp;</em>seeds have the potential to inhibit adipogenesis and obesity. Overall, this study explored the inhibitory effects of&nbsp;<em>Phoenix dactylifera</em>&nbsp;L. seed extract on adipogenesis in 3T3-L1 cells on the molecular level.</div> Adipocyte Differentiation, Adipogenesis, Glucose Homeostasis, Obesity, Phoenix Dactylifera Seed, 3T3-L1 Cell. 14 25 http://rbmb.net/browse.php?a_code=A-10-396-1&slc_lang=en&sid=1 Behrouz Etesami Behroozbiochem@gmail.com 100319475328460017481 100319475328460017481 No Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Sara Ghaseminezhad ghasemi.sarah@yahoo.com 100319475328460017482 100319475328460017482 No Department of Chemistry, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran. Azin Nowrouzi nwr29@yahoo.com 100319475328460017483 100319475328460017483 Yes Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Marzieh Rashidipour M_rashidi80@yahoo.com 100319475328460017484 100319475328460017484 No Department of Environmental Toxicology, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Razieh Yazdanparast ryazdan@ut.ac.ir 100319475328460017485 100319475328460017485 No Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.