Reports of Biochemistry and Molecular Biology

en Molecular Detection of Campylobacter Species: Comparision of 16SrRNA with slyD, cadF, rpoA, and dnaJ Sequencing میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;">Background: Campylobacter spp. are the main cause of human gastroenteritis. The 16SrRNA sequencing is one of fast molecualr method to detect this fastidious. In this study, we compared the sequencing of 16srRNA genewith four housekeeping genestodetect Campylobacter spp. in patients with diarrhea and healthy people. Methods: 60 samples of Campylobacter DNA extracted from stool samples of 30 patients with diarrhea and 30 healthy people were used. In order to detect Campylobacter, we designed primers for proliferation of 16SrRNA, cadF, dnaJ, slyD, and rpoA genes using Primer 3, Mega 4.0 and Blast software. Then the PCR products were sequenced using ABI system. Results: The sequencing showed concordance of PCR-products with deposited sequences in the Gene Bank. Among diarrhea patients, 53.3% of samples were significantly (p< 0.05) positive for slyD and cadF genes and 50% of samples were positive using 16SrRNA, rpoA, and dnaJ genes by PCR assay. The average of sensitivity and specificity were found 53.33% and 83.33%, respectively. Conclusions: Due to various copies of repeated sequences of 16SrRNA gene, analyzing its amplicons on electrophoresis may be more difficult than the slyD and cadF genes. According to our results, among the 5 studied genes; the highest detection rate was related to slyD and cadF genes. Although, dnaJ and rpoA genes, instead of 16SrRNA gene, can be considered as appropriate genes for molecular detection of Campylobacter bacteria.</div> cadF, Campylobacter, Diarrhea, Molecular detection, slyD. 257 263 http://rbmb.net/browse.php?a_code=A-10-496-1&slc_lang=en&sid=1 Elahe Ghorbani marghmaleki 100319475328460017646 100319475328460017646 No Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran. Azam Ahmadi ahmadia22@yahoo.com 100319475328460017647 100319475328460017647 Yes Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran. Mohammad Arjomandzadegan 100319475328460017648 100319475328460017648 No Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran. Majid Akbari 100319475328460017649 100319475328460017649 No Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran. Aysan Karamghoshchi 100319475328460017650 100319475328460017650 No Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran.