en میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> <em>Staphylococcus aureus</em> as a causative agent of hospital-acquired infections has been considered as the primary concern in biomaterial-related infections (BAIs).<br> <br> <strong><em>Methods:</em></strong> Following the purification of polysaccharide intercellular adhesion (PIA) as an efficient macromolecule in biofilm formation in the native condition, recombinant <em>S</em>. <em>epidermidis</em> surface-exposed rSesC protein, with the most homology to clumping factor A (ClfA) in <em>S. aureus</em> was cloned and expressed in a prokaryotic host as well. Fourier transform infrared spectrometry (FTIR) and Western blotting procedure analyzed purified PIA and protein, respectively. Then, the immune response was evaluated by measuring total IgG titers. Moreover, the capacity of Anti-biofilm forming activity of arisen antibodies to a biofilm-forming <em>S. aureus</em> strains was assessed by the semi-quantitative micro-plate procedure.<br> <br> <strong><em>Results:</em></strong> Data showed that the total IgGs were boosted in mice immunized sera. By performing an inhibition assay, the biofilm inhibitory effect of secreted antibodies to test strain was observed. Arisen antibodies against the mixture significantly were more potent than PIA and rSesC, when comparing individual antigens in a biofilm inhibition assay.<br> <br> <strong><em>Conclusions:</em></strong> immunization of mice with mentioned antigens especially a mixture of them, could eliminate the biofilm formation process in <em>S. aureus</em>. Hopefully, this study corresponds to the suggestion that the immunization of mice with PIA and rSesC candidate vaccines could protect against <em>S. aureus</em> infection.</div> PIA, Purification, Staphylococcus aureus, rSesC, Vaccine candidates. 1 12 http://rbmb.net/browse.php?a_code=A-10-137-3&slc_lang=en&sid=1 Bahman Mirzaei dr.bahman.m@gmail.com 100319475328460012703 100319475328460012703 Yes Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences. & Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science. Ryhaneh Babaei 100319475328460012704 100319475328460012704 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences. & Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science. Mohammad Reza Haghshenas goli59@gmail.com 100319475328460012705 100319475328460012705 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences. Mohammad Reza Hagshenas 100319475328460012706 100319475328460012706 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. Fatemeh Mohammadi 100319475328460012707 100319475328460012707 No Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science. Pegah Homayoni 100319475328460012708 100319475328460012708 No Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science. Ebrahim Shafaei e.shafaei1986@yahoo.com 100319475328460012709 100319475328460012709 No Infectious diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.