en میکروب شناسی Microbiology مقالات اصلی Original Article <strong><em>Background:</em></strong> According to the studies, many pathogens function as cofactors interacting with&nbsp;Human papillomavirus in the development of pre-cancer or cancer of the cervix. The aim of this&nbsp;study was to investigate the prevalence rate of Sexually Transmitted Infections (STIs) pathogens&nbsp;including&nbsp; Mycoplasma hominis, Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria&nbsp;gonorrhoeae, Gardnerella vaginalis, and Streptococcus agalactiae in people with HPV and without&nbsp;HPV infection, and frequency rate of these pathogens in high and low risk of HPV.<br> <br> <strong><em>Methods:</em></strong> Cervical samples of 280 women who referred to Tehran west hospitals in Iran, between&nbsp;2019 and 2020, were collected. After DNA extraction of samples, identification of HPV and&nbsp;genotyping was performed, and then, to detect each microorganism, the PCR was carried out with&nbsp;specific primers. Finally, the results were analyzed using descriptive statistics tests.&nbsp;<br> <br> <strong><em>Results:</em></strong> The mean age of patients was 37 years. Two groups of patients were identified based on&nbsp;positivity or negativity of HPV. In HPV-positive group (118 cases), the prevalence of U.&nbsp;urealyticum, M. hominis, N. gonorrhoeae, G. vaginalis, and S. agalactiae was 38 (13%), 7 (62%),&nbsp;5.93%, 19.49%, 0.84% respectively. In HPV-negative group (162 cases), rate of infection with U.&nbsp;urealyticum, M. hominis, N. gonorrhoeae, G. vaginalis, and S. agalactiae was 29.62%, 6.17%,&nbsp;3.08%, 16.04%, 0.61% respectively. Among the two groups, there was only 1 patient with C.&nbsp;trachomatis (0.84%), seen in HPV-positive group.<br> <br> <strong><em>Conclusions:</em></strong> In this study no significant association was found between HPV and bacteria such as&nbsp;G. vaginalis and S. agalactiae, and it was found that C. trachomatis, and especially N. gonorrhoeae&nbsp;are strongly associated with HPV infection. HPV, Sexually transmitted infections, PCR. 387 395 http://rbmb.net/browse.php?a_code=A-10-627-1&slc_lang=en&sid=1 Seyed Mojtaba Mortazavi 100319475328460012527 100319475328460012527 No Student Research Committee, School of Medicine, Bam University of Medical Sciences, Bam, Iran. Amin Tarinjoo 100319475328460012528 100319475328460012528 No Islamic Azad University, science and research branch, Tehran, Iran. Sepideh Dastani 100319475328460012529 100319475328460012529 No Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Majid Niazpour 100319475328460012530 100319475328460012530 No Tehran Azadi Pathobiology Laboratory. Samira Dahaghin sa.dahaghin@gmail.com 100319475328460012531 100319475328460012531 Yes Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Reza Mirnejad* rmirnejad@yahoo.com 100319475328460012532 100319475328460012532 No Molecular Biology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.