Reports of Biochemistry and Molecular Biology

en Clinical Significance of Circulating Serum Levels of sCD95 and TNF-α in Cytoprotection of Cervical Cancer بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC). Methods: Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat cells. Data was scrutinized through statistical analysis. Results: Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC. Conclusions: Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.</div> Apoptosis, sCD95, Jurkat Cells, Tumor Necrosis Factor-alpha, Uterine Cervical Neoplasms. 711 721 http://rbmb.net/browse.php?a_code=A-10-723-1&slc_lang=en&sid=1 Saurabh Kumar Agnihotri 100319475328460012493 100319475328460012493 No Department of Radiotherapy, King George’s Medical University, Lucknow 226 003, India & Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow 226 031, India. Balawant Kumar 100319475328460012494 100319475328460012494 No Division of Toxicology & Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow 226 031, India. Ankita Jain 100319475328460012495 100319475328460012495 No Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow 226 031, India. Anjali Anjali 100319475328460012496 100319475328460012496 No Division of Toxicology & Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow 226 031, India & Department of Obstetrics & Gynaecology, King George’s Medical University, Lucknow 226 003, India. Mahendra Pal Singh Negi 100319475328460012497 100319475328460012497 No Division of Toxicology & Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow 226 031, India. Rekha Sachan 100319475328460012498 100319475328460012498 No Department of Obstetrics & Gynaecology, King George’s Medical University, Lucknow 226 003, India. Madan Lal Brahma Bhatt 100319475328460012499 100319475328460012499 No Department of Radiotherapy, King George’s Medical University, Lucknow 226 003, India. Rekha Sachan 100319475328460012500 100319475328460012500 No Department of Obstetrics & Gynaecology, King George’s Medical University, Lucknow 226 003, India. Madan Lal Brahma Bhatt 100319475328460012501 100319475328460012501 No Department of Radiotherapy, King George’s Medical University, Lucknow 226 003, India. Raj Kamal Tripathi 100319475328460012502 100319475328460012502 No Division of Toxicology & Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow 226 031, India & Department of Obstetrics & Gynaecology, King George’s Medical University, Lucknow 226 003, India. Monika Sachdev monika@cdri.res.in. 100319475328460012503 100319475328460012503 Yes Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow 226 031, India & Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India.