en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> The available evidence has increasingly demonstrated that a combination of genetic and&nbsp;epigenetic factors, such as DNA methylation, could be considered as causing leukemia. Epigenetic changes&nbsp;and methylation of the suppressor of the cytokine signaling 1 promoter (SOCS1) CpG region silence SOCS1&nbsp;expression in cancer. In the current study, we evaluated the impact of epigallocatechin gallate (EGCG) and&nbsp;RG108 on SOCS1 promoter methylation and expression in U937 cells.<br> <br> <strong><em>Methods: </em></strong>In the current study, U937 leukemic cells were treated with EGCG and RG108 for 12, 24,&nbsp;48, and 72 h and SOCS1 promoter methylation and its expression were measured by methylationspecific&nbsp;PCR (MSP) and quantitative real-time PCR, respectively.&nbsp;<br> <br> <strong><em>Results:</em></strong> The outcomes indicated that the SOCS1 promoter is methylated in U937 cells, and treatment of&nbsp;these cells with either EGCG or RG108 reduced its methylation. Moreover, we observed that SOCS1&nbsp;expression was significantly upregulated in a time-dependent manner by both EGCG and RG108 in U937<br> cells compared with control cells. In the RG108-treated group at 12, 24, 48, and 72 h, SOCS1 expression&nbsp;was upregulated by 1, 4.2, 16.6, and 32.6 -fold respectively, and in the EGCG-treated group, by 0.5, 3.2,&nbsp;10.8, and 22.3 -fold, respectively.<br> <br> <strong><em>Conclusions: </em></strong>Treatment with either EGCG or RG108 reduced SOCS1 promoter methylation and&nbsp;increased SOCS1 expression in U937 cells in a time-dependent manner, which may play a role in&nbsp;leukemia therapy.</div> &nbsp; SOCS1, RG108, EGCG, Leukemia, DNA Methylation 455 461 http://rbmb.net/browse.php?a_code=A-10-767-1&slc_lang=en&sid=1 Mohsen Alizadeh 100319475328460012556 100319475328460012556 No Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran. Amirhossein Nafari 100319475328460012557 100319475328460012557 No Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Ali Safarzadeh 100319475328460012558 100319475328460012558 No Department of animal science ,Lorestan Agricultural and Natural Resources Research and Education Center, Iran Saeed Veiskarami 100319475328460012559 100319475328460012559 No Department of animal science ,Lorestan Agricultural and Natural Resources Research and Education Center, Iran Mohammad Almasian 100319475328460012560 100319475328460012560 No School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Ali Asghar Kiani kiani.a@lums.ac.ir. 100319475328460012561 100319475328460012561 Yes Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran