en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> The aim of present study is to asset the IL-2 promoter gene (SNP -475) as a candidate gene&nbsp;for multiple sclerosis (MS) susceptibility.<br> <br> <strong><em>Methods:</em></strong> This study included 70 patients with relapsing &ndash; remitting multiple sclerosis (RRMS) and 50&nbsp;healthy controls. Following the extraction of genomic DNA from peripheral blood, frequency of genotypes&nbsp;and alleles of SNP -475 was calculated using Restriction fragment length polymorphism-polymer chain<br> reaction (RFLP-PCR) and then the results were analyzed statistically.<br> <br> <strong><em>Results:</em></strong> The results revealed the unusual ratio for the heterozygous (AT) was 1.6972 indicating that&nbsp;heterozygous patients were at higher risk of multiple sclerosis than wild homozygous (AA), and&nbsp;homomutant (TT). The results show protective role for - 475 IL-2 promoter among individuals with multiple<br> sclerosis, (O.R: 0.4872; C.I. 95%: 0.1617- 1.4680) and (O.R: 0.9275; C.I. 95%: 0.2476 - 3.4745) for both&nbsp;AA and TT genotypes, respectively.<br> <br> <strong><em>Conclusions: </em></strong>Our results showed that in this population of Iraqi patients, the AT genotype / A allele of -475&nbsp;IL-2 promoter gene SNP may include attributed factors for MS predisposition.</div> IL-2, Multiple sclerosis, PCR-RFLP SNP. 83 88 http://rbmb.net/browse.php?a_code=A-10-776-1&slc_lang=en&sid=1 Thekra Abdulaali Abed 100319475328460012347 100319475328460012347 No Department of Biology, College of Sciences, University of Babylon, Iraq. Anwar Ali Abdulla alhussainybio@yahoo.com. 100319475328460012348 100319475328460012348 Yes Department of Biology, College of Sciences, University of Babylon, Iraq.