en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Breast Cancer (BC), the second leading cause of cancer mortality after lung cancer and varied&nbsp;across the world due to genetic and environmental factors. In this study, we evaluated the interaction&nbsp;between the polymorphisms in genes encoding enzymes of folate metabolism: methylenetetrahydrofolate&nbsp;reductase (MTHFR), methionine synthesis reductase (MTR) with the BC prognostic factors.<br> <br> <strong><em>Methods:</em></strong> This study was conducted on 160 Egyptian subjects, 60 controls and 100 cases. Sequencing,&nbsp;RFLP analysis in addition to statistical analysis including Chi‐squared test, haplotype analysis was used to&nbsp;evaluate associations with BC risk and its clinicopathological parameters. Odds ratios (ORs) and 95%&nbsp;confidence intervals (CIs) were calculated using unconditional logistic regression.<br> <br> <strong><em>Results:</em></strong> Strong significant association with breast cancer risk was observed for the haplotype (T-C-G) of&nbsp;MTHFR C677T/ MTHFR A1289C and MTRA2576G and hormonal receptor expression (ER-/PR-&nbsp;/HER2+), bigger and advanced tumor and metastatic lymph nodes. However, no significant difference was<br> observed for age.<br> <br> <strong><em>Conclusions:</em></strong> The combination of SNPs from MTHFR and MTR genes has a more synergistically genetic&nbsp;effect on BC disease progression. These SNPs could be used as tumor aggressiveness markers among&nbsp;Egyptian females with BC and could help in saving money and time.</div> Breast cancer, Methionine synthesis reductase, MTHFR, PCR-RFLP, SNPs. 89 101 http://rbmb.net/browse.php?a_code=A-10-778-1&slc_lang=en&sid=1 Moataza Moataza Hassan Omran moataza13@yahoo.com. 100319475328460012349 100319475328460012349 Yes Microbial Biotechnology Department, Genetic Engineering Division, National Research Centre, Cairo, Egypt (12622). Basma El-Sayed Fotouh 100319475328460012350 100319475328460012350 No Microbial Biotechnology Department, Genetic Engineering Division, National Research Centre, Cairo, Egypt (12622). Wafaa Ghoneim Shousha 100319475328460012351 100319475328460012351 No Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt (11795). Abeer Ismail 100319475328460012352 100319475328460012352 No Department of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Giza, Egypt (12613). Shimaa Shawki Ramadan 100319475328460012353 100319475328460012353 No Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt (11795).