en ایمنی شناسی Immunology <p style="text-align: justify;"><em><strong>Background: </strong></em>Diagnosis of food allergy is difficult in children. Food allergies are diagnosed using several methods that include medical histories, clinical examinations, skin prick and serum-specific immunoglobulin E (IgE) tests, radio-allergosorbent test (RAST), food challenge, and supervised elimination diets. In this study we evaluated allergies to cow&#39;s milk, egg, peanut, and fish in children with suspected food allergies with skin prick tests and serum and feces RAST.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Forty-one children with clinical symptoms of food allergies were enrolled in the study. Skin prick tests and serum and fecal RAST were performed and compared with challenge tests.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> The most common sites of food allergy symptoms were gastrointestinal (82.9%) and skin (48.8%). 100% of the patients responded to the challenge tests with cow&rsquo;s milk, egg, peanut, and fish. 65% of the patients tested positive with the skin prick test, 12.1% tested positive with serum RAST, and 29.2% tested positive with fecal RAST.</p> <p style="text-align: justify;"><em><strong>Conclusions:</strong></em> The skin prick test was more sensitive than serum or fecal RAST, and fecal RAST was more than twice as sensitive as serum RAST.</p> Food Hypersensitivity, Radioallergosorbent test, Sensitivity, Specificity 98 103 http://rbmb.net/browse.php?a_code=A-10-1-56&slc_lang=en&sid=1 Hamid Reza Kianifar 10031947532846006141 10031947532846006141 No Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran - Clinical Research Development Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Alireza Pourreza 10031947532846006142 10031947532846006142 No Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Farahzad Jabbari Azad Jabbarif@mums.ac.ir 10031947532846006143 10031947532846006143 Yes Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Hadis Yousefzadeh 10031947532846006144 10031947532846006144 No Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Fatemeh Masomi 10031947532846006145 10031947532846006145 No Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran