en ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;"><em><strong>Background:</strong></em> inflammatory chemokines such as CCL2 and CCL5 are involved in the progress of&nbsp;osteoarthritis. Crocin with antioxidant and anti-inflammatory properties can reduce the symptoms of&nbsp;osteoarthritis (OA). This study was performed investigate the effect of Krocina&trade;, on the gene expressions&nbsp;and plasma levels of CCL2 and CCL5 in OA patients.<br> <br> <strong><em>Methods:</em></strong> The study included 35 patients that were randomized in the KrocinaTM and placebo groups. The&nbsp;intervention was Krocina&trade; 15 mg daily for four months. Clinical and paraclinical parameters were&nbsp;measured. CCL2 and CCL5 genes expression and plasma levels were determined using the SYBR Green<br> Real-Time RT-PCR and Enzyme-linked Immunosorbent Assay (ELISA) techniques.<br> <br> <em><strong>Results:</strong></em> The C-reactive protein (CRP) value in the KrocinaTM group and the visual analogue scale (VAS)&nbsp;value in the KrocinaTM and placebo groups decreased significantly after the intervention. The gene&nbsp;expression of CCL2 in the KrocinaTM and placebo groups decreased significantly. On the contrary, the gene&nbsp;expression of CCL5 in the KrocinaTM and placebo groups increased significantly. Moreover, the plasma&nbsp;levels of CCL2 in the KrocinaTM and placebo groups decreased meaningfully. There was no difference&nbsp;regarding the plasma levels of CCL5 within the Krocina&trade; and placebo groups before and after the&nbsp;intervention in either of the groups.<br> <br> <strong><em>Conclusions:</em></strong> Administration of Krocina&trade; reduced the clinical signs of inflammation and CRP and VAS&nbsp;value. Also, Krocina&trade; significantly decreased the plasma levels and gene expression of CCL2 in&nbsp;osteoarthritis patients.</div> CCL2, CCL5, Krocina™, Osteoarthritis. 10 19 http://rbmb.net/browse.php?a_code=A-10-823-2&slc_lang=en&sid=1 Samaneh Beiki Beiki941@gmail.com 100319475328460012306 100319475328460012306 No Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran & Immunology Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Jalil Tavakkol-Afshari Tavakolaj@mums.ac.ir 100319475328460012307 100319475328460012307 No Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Zhaleh Shariati-Sarabi Shariatij@mums.ac.ir 100319475328460012308 100319475328460012308 No Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran & Internal Medicine Department, Imam Reza hospital, Mashhad University of Medical Sciences, Mashhad, Iran. Javad Poursamimi javadpoursamimi@gmail.com 100319475328460012309 100319475328460012309 No Department of Immunology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran. Arezoo Gowhari Shabgah gohariarezoo@yahoo.com 100319475328460012310 100319475328460012310 No School of Medicine, Bam University of Medical Sciences, Bam, Iran. Mojgan Mohammadi mohammadimzh@mums.ac.ir 100319475328460012311 100319475328460012311 Yes Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.