en بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background: </em></strong>Oxidative stress is defined as the condition in which balance between the synthesis and&nbsp;detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of&nbsp;intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and<br> specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand&nbsp;White (NZW) rabbits.<br> <br> <strong><em>Methods:</em></strong> Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting&nbsp;(PF) groups. The controls were fed ad lib. IF and PF groups were fasted for 16 and 40 hours, respectively,&nbsp;followed by eight hours of non-fasting, for six days and were sacrificed on the 7th day. One hundred mg of&nbsp;heart, brain, and kidney tissues were homogenized in 1 ml of phosphate-buffered saline. MDA, carbonyl,&nbsp;GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and&nbsp;post hoc test.<br> <br> <strong><em>Results:</em></strong> In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH&nbsp;was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was&nbsp;significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was<br> significantly less in the PF than in the control group.&nbsp;<br> <br> <em><strong>Conclusions:</strong></em> The effect of fasting on oxidative stress in various organs showed various responses, however&nbsp;fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.</div> Fasting, Oxidative stress, Vital organs, Rabbit 190 199 http://rbmb.net/browse.php?a_code=A-10-860-1&slc_lang=en&sid=1 Novi Silvia Hardiany novi.silvia@ui.ac.id. 100319475328460012165 100319475328460012165 Yes Department of Biochemistry & Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia & Center of Hypoxia & Oxidative Stress Studies, Department of Biochemistry & Molecular. Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Adrian Prasetya Karman 100319475328460012166 100319475328460012166 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Aisha Safa Putri Calista 100319475328460012167 100319475328460012167 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Budi Gittanaya Anindyanari 100319475328460012168 100319475328460012168 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Daniell Edward Rahardjo 100319475328460012169 100319475328460012169 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Puti Retasya Novira 100319475328460012170 100319475328460012170 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Rania Rifdah Taufiq 100319475328460012171 100319475328460012171 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Shofiyya Imtiyaz 100319475328460012172 100319475328460012172 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. Radiana Dhewayani Antarianto 100319475328460012173 100319475328460012173 No Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.