en میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Human Adenovirus species D (HAdV-D) was common human viral pathogen&nbsp;especially in eye infection, consists of several types of which HAdV-D8, -D19 and &ndash;D37 were&nbsp;common in eye infection. This study includes detection of HAdV-D types implicated in conjunctivitis&nbsp;based on L2 (Penton protein) gene similarity.<br> <br> <strong><em>Methods:</em></strong> Conjunctival swabs were collected from Keratoconjunctivitis patients as eye infection&nbsp;related to adenovirus. Viral nucleic acids were extracted and specific primer pairs for HAdV-D L2&nbsp;gene (encoding for penton base protein) was used to amplify the target gene and only positive samples&nbsp;were sent to sequencing.<br> <br> <strong><em>Results:</em></strong> The results revealed that only 6 samples give positive results for L2 gene amplification and&nbsp;then sent for sequencing for L2 (penton protein) gene-based typing. The results show that 4 local&nbsp;isolates (S1, S2, S3, S6) were similar to HAdV-D8 and 2 local isolates (S4, S5) were similar to HAdVD20.<br> Also the results display that the HAdV-37, prominent HAdV-D type of human eye infection,&nbsp;may be variant of HAdV-D20 due to that six variation were seen in S4and S5 local isolates nucleotide&nbsp;sequence in relation to HAdV-D37: T>C at position 14364, A>C at position 14411, T>C at position&nbsp;14427, C>A at position 14448, G>A at position 14540 and T>C at position 14617, leading to only 2&nbsp;amino acid change in resulted penton protein: T (Threonine) instead of K (Lysine) at position 204&nbsp;and N (Asparagine) instead of D (Aspartic acid) at position 247.<br> <br> <strong><em>Conclusions:</em></strong> The current study concludes the possibility of implication of HAdV-D20 in eye&nbsp;infections especially conjunctivitis.</div> HAdV-D8, HAdV-D20, HAdV-D37, conjunctivitis, Iraq 176 181 http://rbmb.net/browse.php?a_code=A-10-779-4&slc_lang=en&sid=1 Masar Riyadh Rashid Al-Mousawi 100319475328460012381 100319475328460012381 No Department of Medical Microbiology, Collage of medicine, University of Kerbala, Iraq. May Mohammed Ali 100319475328460012382 100319475328460012382 No Department of Medical Microbiology, Collage of medicine, University of Kerbala, Iraq. Noor Salman Kadhim Al-Khafaji 100319475328460012383 100319475328460012383 No Department of Biology, college of science, University of Babylon, Iraq. Hussein Oleiwi Muttaleb Al-Dahmoshi dr.dahmoshi83@gmail.com. 100319475328460012384 100319475328460012384 Yes Department of Biology, college of science, University of Babylon, Iraq.