en میکروب شناسی Microbiology <p style="text-align: justify;"><em><strong>Background: </strong></em>Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the <em>Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). </em></p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> First, an <em>hspX</em> fragment was amplified by PCR and cloned into plasmid pcDNA3.1(+) and recombinant vector was confirmed.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> A 435 bp hspX fragment was isolated. The fragment was 100% homologous with <em>hspX</em> of M. tuberculosis strain H37Rv in GenBank.</p> <p style="text-align: justify;"><em><strong>Conclusions:</strong></em> In this study, the cloning vector pcDNA3.1(+), containing a 435-bp hspX fragment of <em>M. tuberculosis</em>, was constructed. This could be used as a DNA vaccine to induce immune responses in animal models in future studies.</p> Cloning, DNA vaccine, hspX, Mycobacterium tuberculosis 46 50 http://rbmb.net/browse.php?a_code=A-10-1-64&slc_lang=en&sid=1 Atieh Yaghoubi 10031947532846006176 10031947532846006176 No Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Ehsan Aryan 10031947532846006177 10031947532846006177 No Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Hosna Zare 10031947532846006178 10031947532846006178 No Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Shadi Alami 10031947532846006179 10031947532846006179 No Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Roghayeh Teimourpour 10031947532846006180 10031947532846006180 No Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. Zahra Meshkat meshkatz@mums.ac.ir 10031947532846006181 10031947532846006181 Yes Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran