Reports of Biochemistry and Molecular Biology

en Evaluation of Caffeine Ingested Timing on Endurance Performance based on CYP1A2 rs762551 Profiling in Healthy Sedentary Young Adults زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Caffeine is generally suggested to increase VO2max in endurance performance. Nevertheless, the response to caffeine ingestion does not seem to be uniform across individuals. Therefore, caffeine ingested timing on endurance performance based on the type of CYP1A2 single nucleotide polymorphism rs762551, that were classified as fast and slow metabolizers, need to be evaluated. Methods: Thirty participants participated in this study. DNA was obtained from saliva samples and genotyped using polymerase chain reaction-restriction fragment length polymorphism. Each respondent completed beep tests under three treatments blindly: placebo, 4 mg/kg body mass of caffeine one hour, and two hours before test.   Results: Caffeine increased estimated VO2max in fast metabolizers (caffeine=29.39±4.79, placebo=27.33±4.02, p<0.05) and slow metabolizers (caffeine=31.25±6.19, placebo=29.17±5.32, p<0.05) in one hour before test. Caffeine also increased estimated VO2max in fast metabolizers (caffeine=28.91±4.65, placebo=27.33±4.02, p<0.05) and slow metabolizers (caffeine=32.53±6.68, placebo=29.17±5.32, p<0.05) in two hour before test. However, for slow metabolizers, the increasing was greater when caffeine was administered two hours before test (slow=3.37±2.07, fast=1.57±1.62, p<0.05). Conclusions: Genetic variance may affect the optimal caffeine ingestion timing, sedentary individuals who want to enhance their endurance performance may ingest caffeine 1 hour before exercise for fast metabolizers and 2 hours before exercise for slow metabolizers.</div> Caffeine, CYP1A2, Performance Enhancer, Sedentary, VO2max. 663 671 http://rbmb.net/browse.php?a_code=A-10-1106-1&slc_lang=en&sid=1 Yones Guntoro Aji 100319475328460016839 100319475328460016839 No Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia. Stella Melita 100319475328460016840 100319475328460016840 No Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia. Rendy Dijaya 100319475328460016841 100319475328460016841 No Nutrifood Research Center, PT. Nutrifood Indonesia, Jakarta 13920, Indonesia. Dionysius Subali dionysius.subali@atmajaya.ac.id. 100319475328460016842 100319475328460016842 Yes Department of Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University, of Indonesia, Jakarta 12930, Indonesia. Felicia Kartawidjajaputra 100319475328460016843 100319475328460016843 No Nutrifood Research Center, PT. Nutrifood Indonesia, Jakarta 13920, Indonesia. Antonius Suwanto 100319475328460016844 100319475328460016844 No Departement of Biology, Faculty of Mathematics and Natural Sciences, IPB University.