en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Background:</span></span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">A critical role has been known for<b> </b>lncRNAs<b> </b>in the initiation and development of cancers. Therefore, lncRNAs have been reported as the possible biomarkers in relation to the diagnosis and therapy of malignancies. This project examined the change in CYTOR lncRNA expression in human cervical cancer samples as compared with adjacent healthy ones.</span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Methods:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">We provided<b> </b>one hundred fifteen pairs of tumorous and adjacent healthy tissue specimens of cervical cancer patients. RNAs were isolated from tissue specimens and cDNAs were synthesized. We considered quantitative Real-time PCR (qRT-PCR) to examine the expression levels of CYTOR lncRNA. In addition, the biomarker activity of CYTOR and the associations between the lncRNA and clinicopathological characteristics were evaluated.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Results:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The significant increased expression of CYTOR was obtained in cancerous samples as compared with non-cancerous ones (<i>P< </i>0.0001). A significant correlation was indicated between CYTOR expression and the squamous subtype of cervical cancer (<i>p</i>=0.046). The receiver operating characteristic (ROC) curve-related AUC (area under the curve), specificity, and sensitivity were calculated 0.88, 81.74%, and 80%, respectively, which may introduce CYTOR as a potential biomarker.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">CYTOR may be an effective oncogene and biomarker in cervical cancer cases given its increased expression in human cervical cancer tissues.</span></span></span></span></span></span></span></span></span></div> Biomarker, Cervical cancer, CYTOR, qRT-PCR, lncRNA. 120 126 http://rbmb.net/browse.php?a_code=A-10-1212-1&slc_lang=en&sid=1 Ali Rajabi 100319475328460017848 100319475328460017848 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Narges Dastmalchi 100319475328460017849 100319475328460017849 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Neda Shokri 100319475328460017850 100319475328460017850 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Samaneh Tayefeh-Gholami 100319475328460017851 100319475328460017851 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Seyyed Mohammad Yaghobi 100319475328460017852 100319475328460017852 No Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. Reza Safaralizadeh Safaralizadeh@tabrizu.ac.ir. 100319475328460017853 100319475328460017853 Yes Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.