en بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Background:</span></span></span></span></i></b><b><i> </i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Colorectal cancer is a heterogeneous disease that leads to metabolic disorders due to multiple upstream genetic and molecular changes and interactions. The development of new therapies, especially herbal medicines, has received much global attention. <i>Dorema ammoniacum</i> is a medicinal plant. Its gum is used in healing known ailments. Studying metabolome profiles based on nuclear magnetic resonance 1HNMR as a non-invasive and reproducible tool can identify metabolic changes as a reflection of intracellular fluxes, especially in drug responses. </span></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">This study aimed to investigate the anti-cancer effects of different gum extracts on metabolic changes and their impact on gene expression in HT-29 cell.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Methods:</span></span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Extraction of <i>Dorema ammoniacum</i><b> </b>gum with hexane, chloroform, and dichloromethane organic solvents was performed. Cell inhibition growth percentage and IC₅₀ were assessed. Following treating the cells with dichloromethane extract, <i>p53, APC, </i>and<i> KRAS</i> gene expression were determined. 1HNMR spectroscopy was conducted. Eventually, systems biology software tools interpreted combined metabolites and genes simultaneously.</span></span></span><br> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Results:</span></span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The lowest determined IC₅₀ concentration was related to dichloromethane solvent, and the highest was hexane and chloroform. The expression of the <i>KRAS</i> oncogene gene decreased significantly after treatment with dichloromethane extract compared to the control group, and the expression of tumor suppressor gene <i>p53</i> and <i>APC</i> increased significantly. Most gene-altered convergent metabolic phenotypes.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.3pt">This study&#39;s results indicate that the dichloromethane solvent of <i>Dorema ammoniacum</i><b> </b>gum exhibits its antitumor properties by altering the expression of genes involved in HT-29 cells and the consequent change in downstream metabolic reprogramming.</span></span></span></span></span></span></span></span></span></span></div> APC, HT-29 cell, KRAS, Metabolomics, Nuclear Magnetic Resonance, P53. 127 135 http://rbmb.net/browse.php?a_code=A-10-1235-1&slc_lang=en&sid=1 Elham Ghodousi-Dehnavi 100319475328460017854 100319475328460017854 No Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran. Mohammad Arjmand arjmand1@yahoo.com 100319475328460017855 100319475328460017855 Yes Metabolomics Lab. Department of Biochemistry, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran. Ziba Akbari 100319475328460017856 100319475328460017856 No Metabolomics Lab. Department of Biochemistry, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran. Mansour Aminzadeh Bukani 100319475328460017857 100319475328460017857 No Metabolomics Lab. Department of Biochemistry, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran. Reza Haji Hosseini 100319475328460017858 100319475328460017858 No Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran.