Reports of Biochemistry and Molecular Biology

en Impact of Cerium Oxide Nanoparticles on Metabolic, Apoptotic, Autophagic and Antioxidant Changes in Doxorubicin-Induced Cardiomyopathy: Possible Underlying Mechanisms بیوشیمی Biochemistry مقالات اصلی Original Article <div class="WordSection1"> <div style="text-align: justify;">Background: In the current study, the effects of cerium oxide nanoparticles (nanocerium; NC) on doxorubicin (DOX)-induced cardiomyopathy and its possible underlying mechanisms were addressed. Methods: 32 adult male rats were allocated into 4 groups; i) control group, ii) NC group; rats received NC (0.2 mg/kg, i.p., daily), iii) DOX group; rats received DOX 4 mg/kg (2 injections with a 14-day interval), and iv) DOX+NC group as DOX but rats received NC. At the end of the experiment, ECG and ECHO recordings and assessments of the levels of cardiac enzymes (CK-MB, LDH), and myocardial oxidative stress (MDA, catalase, and GSH), the expression of LC3 and beclin1 (markers of autophagy), caspase3 (marker of apoptosis) by immunohistochemistry, the expression of acetyl-CoA carboxylase alpha (ACCA) by PCR, and 5’adenosine monophosphate-activated protein kinase (AMPK) levels in the heart tissues were performed. Results: The DOX group displayed a prolonged corrected QT interval, an increase in cardiac enzymes (CK-MB and LDH), myocardial oxidative stress (high MDA with low catalase and GSH), expression of ACCA, caspase-3, beclin1, and LC3 in myocardial tissues, with reduction in myocardial AMPK levels, and myocardial contractility (low ejection fraction, and fractional shortening). On the other hand, administration of NC with DOX resulted in significant improvement of all studied parameters. Conclusions: NC offers a cardioprotective effect against DOX-induced cardiomyopathy. This effect might be due to its antioxidant and antiapoptotic effects as well as to the modulation of autophagy and metabolic dysfunctions induced by DOX in the heart tissues. </div> <div align="center" style="text-align:center"> <hr align="center" noshade="noshade" size="2" style="color:#0f243e" width="100%" ></div> </div> <div style="text-align: justify;"><div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div></div> Autophagy, Cardiomyopathy, Cerium oxide nanoparticles, Doxorubicin, Echocardiography, Oxidative stress. 495 511 http://rbmb.net/browse.php?a_code=A-10-1130-3&slc_lang=en&sid=1 Shorouk Elmorshdy Elsaeed Mohammed Elmorshdy 100319475328460018620 100319475328460018620 No Medical physiology department, Faculty of Medicine, Mansoura University, Egypt. Gehan Ahmed Shaker 100319475328460018621 100319475328460018621 No Medical physiology department, Faculty of Medicine, Mansoura University, Egypt. Zienab Helmy Eldken 100319475328460018622 100319475328460018622 No Medical physiology department, Faculty of Medicine, Mansoura University, Egypt & Department of Basic Medical Sciences, Ibn Sina University for Medical Sciences, Amman11104, Jordan. Mahmoud Abdelbadie Salem 100319475328460018623 100319475328460018623 No Department of cardiology, Mansoura Faculty of Medicine, Mansoura university, Egypt. Amira Awadalla 100319475328460018624 100319475328460018624 No Center of Excellence for Genome and Cancer Research, Urology and Nephrology Center, Mansoura University, Mansoura, 35516, Egypt. Hany Mahmoud Abdel Shakour 100319475328460018625 100319475328460018625 No Department of cardiology, Mansoura Faculty of Medicine, Mansoura university, Egypt. Mohammed Elmahdy El Hosiny Sarhan 100319475328460018626 100319475328460018626 No Medical physiology department, Faculty of Medicine, Mansoura University, Egypt. Abdelaziz Mohamed Hussein menhag@mans.edu.eg@yahoo.com 100319475328460018627 100319475328460018627 Yes Medical physiology department, Faculty of Medicine, Mansoura University, Egypt.