en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div class="WordSection1"> <div style="text-align: justify;"><span style="page:WordSection1"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Hypertrophic scar (HS) is a cutaneous condition results from abnormal wound healing following deep tissue injury. To date, there is no optimal treatment for this skin disorder. Catechins possess anti-inflammatory, antioxidant and anti-fibrotic properties. In this study we investigated the effects of catechin hydrate (CH) in rabbit ear model of HS.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Methods:</span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> A&nbsp;rabbit ear model of hypertrophic scar was set up. Ten New Zealand white rabbit were divided into 5 equal groups: non-treatment group, vehicle control, treated with intralesional injection of dimethyl sulfoxide (DMSO), and test groups, received intralesional injection of CH/DMSO solution at concentration of 0.25, 1.25 and, 2.5 mg/ml, respectively. The treatments were initiated </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">35 days after wounding once a </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">week for </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">4 </span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">weeks</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm; &rlm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> The scar elevation index (SEI) and the epidermal thickness index (ETI) were measured using Hematoxylin and Eosin (H&E) </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">staining and the amount of collagen deposition were determined after Masson</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&#39; </span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">trichrome staining</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">. </span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">In addition</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">, the enzyme-linked immunosorbent assay (ELISA) </span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;&rlm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">method was used to determine the levels of </span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">type І </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">and ІІІ collagen and matrix metalloproteinase </span></span></span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&rlm;</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&lrm;</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">1 (MMP1) in scar tissues.<br> <b><i></i></b></span></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results:</span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> CH improved abnormal scarring at concentrations of 1.25 and 2.5 mg/ml and significantly (P<0.001) reduced the SEI and ETI. The levels of collagen type І and type ІІІ, and total collagen deposition were significantly (P<0.05) decreased in scar tissues of CH treated groups and no significant effect on MMP1 levels.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Our findings demonstrated that CH has the potential for the treatment of HSs.</span></span></span></span></span></span></span></span></span></span></div> </div> <div style="text-align: justify;"><div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div></div> Catechin, Collagen, Hypertrophic scar, Rabbit, Wound healing. 13 22 http://rbmb.net/browse.php?a_code=A-10-1448-1&slc_lang=en&sid=1 Sina Goudarzi Afshar 100319475328460020272 100319475328460020272 No Department of Pharmacology &Toxicology, School of Pharmacy, Hamadan University of ‎Medical Sciences, Hamadan, Iran. Pari Tamri p.tamri@umsha.ac.ir 100319475328460020273 100319475328460020273 Yes Department of Pharmacology &Toxicology, School of Pharmacy, Hamadan University of ‎Medical Sciences, Hamadan, Iran. Alireza Nourian 100319475328460020274 100319475328460020274 No Department of Laboratory Sciences, Faculty of Para-Veterinary Sciences, Bu-Ali Sina ‎University, Hamadan, Iran. Ayoub Mahmoudi 100319475328460020275 100319475328460020275 No Department of Pharmacology &Toxicology, School of Pharmacy, Hamadan University of ‎Medical Sciences, Hamadan, Iran.