en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Background:</span></span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">The green synthesis of nanoparticles through algae-mediated processes offers an eco-friendly, cost-effective, and scalable approach for producing nanomaterials with potential applications in cancer therapy. The present study investigated the algae-mediated green synthesis of dextran-coated titanium oxide nanoparticles (TiO₂NPs) and evaluated their cytotoxic effects against MCF-7 breast cancer cells.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Methods: </span></span></span></span></i></b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Chlorella vulgaris</span></span></span></span></i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> was isolated and identified. The polymerase chain reaction (PCR)-amplification of the 18S ribosomal RNA gene was used to confirm the isolate. Dextran from <i>C. vulgaris</i> was used to prepare coated TiO₂NPs, characterized using three techniques. The cytotoxicity of the dextran-coated TiO₂NPs was evaluated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay on MCF7- breast cancer cells at various concentrations (25, 50, and 75%) and exposure times (24, 48, and 72 hours). The bioactive compounds in the algal extract were also identified by gas chromatography-mass spectrometry (GC-MS).</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Results</span></span></span></span></i></b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">: Chlorella vulgaris</span></span></span></span></i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> was successfully isolated as confirmed by the 345-bp PCR-amplified fragment. The characterization of the TiO₂NPs confirmed the successful nanoparticle formation. A cluster of nanocrystalline particles had an average diameter of 71.44 nm. Compositional analysis revealed 15.85%&nbsp;atomic percentage for titanium. The dextran-coated TiO2NPs exhibited an impressive cytotoxicity rate of up to 99% at optimal concentration (25%) and exposure time (48 hours). Additionally, GC-MS analysis identified bioactive compounds in the algal extract, such as fatty acids, which may contribute to the observed anticancer effects.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Conclusion: </span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">The study demonstrated the potential of algae-mediated TiO₂NPs in cancer co-therapy, enhancing treatment effectiveness and reducing the side effects of traditional therapies.</span></span></span></span></span></span></span></span></span></div> Anaphalisoic acid, Capping, Chlorella vulgaris, Cytotoxicity, rRNA gene. 358 367 http://rbmb.net/browse.php?a_code=A-10-1601-1&slc_lang=en&sid=1 Raghad Jasim Fayyad raghadjasim@uomustansiriyah.edu.iq. 100319475328460020685 100319475328460020685 Yes Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq. Entkhab Muhsen Abed Ali Alanisi 100319475328460020686 100319475328460020686 No Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq. Alaa Naseer Mohammed Ali Mohammed Ali alaa2024mu@hotmail.com 100319475328460020687 100319475328460020687 No Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.