Reports of Biochemistry and Molecular Biology

en Anticancer Effects of Escherichia Coli-Derived Outer Membrane Vesicles Against Colorectal Cancer میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;">Background: One of the deadliest cancers in the world, colorectal cancer has a dismal prognosis and a poor response to therapy. It was suggested that outer membrane vesicles (OMVs) produced by Escherichia coli (E. coli) are a powerful inducer of inflammation in intestinal epithelial cells. This research aimed to determine the anticancer potential of E. coli-derived OMVs using a colorectal cancer model. Methods: Five distinct E. coli strains were collected for this study. Their OMVs were then isolated and characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). The effects of E. coli-derived OMVs on colorectal cancer were evaluated in vitro and in vivo using a colorectal tumor model in nude mice. Results: Obtained results showed that E. coli probiotic strains released spherical-shaped vesicles ranging from 5 to 200 nm. E. coli-derived OMVs showed that in the untreated group, a large portion of the tumor tissue continued to grow, with only a few cells undergoing apoptosis. Conversely, the OMV-treated group exhibited a higher number of apoptotic cells, highlighting the anticancer effects of E. coli-derived OMVs in colorectal cancer. Conclusion: These results demonstrated that E. coli-derived OMVs can be employed as a potential treatment for colorectal cancer with minimal adverse effects. Mechanistic studies indicate that these vesicles may promote apoptosis and inhibit cell proliferation, supporting their therapeutic potential. </div> CT-26, Colorectal cancer, E. coli, OMVs. 438 444 http://rbmb.net/browse.php?a_code=A-10-1670-1&slc_lang=en&sid=1 Marjan Ghiyasvand 100319475328460020652 100319475328460020652 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Ardeshir Hesampour Ardeshir.Hesampour@iau.ac.ir. 100319475328460020653 100319475328460020653 Yes Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Hossein Dabiri 100319475328460020654 100319475328460020654 No Microbiology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Javad Arasteh 100319475328460020655 100319475328460020655 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.