Reports of Biochemistry and Molecular Biology

en Intermittent Fasting Attenuates Adriamycin-induced Hepatoxicity in Rats: Possible Role for Nrf2/HO-1 and LC3/Sirt1 Pathways بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: The current study was designed to examine, whether intermittent fasting can ameliorate the liver damage induced by adriamycin (ADR) in rats, as well as its possible underlying mechanisms. Methods: Forty male Sprague Dawley rats were allocated into 4 equal groups, control, fasting, ADR and ADR+ Fasting groups. At the end of the experiment (eight weeks after ADR administration), blood samples were collected for the measurement of ALT, AST, and albumin, and liver tissues were harvested for biochemical analyses of oxidative stress markers (AMD, GSH and Catalase). Real-time PCR was performed for NRF2 and HO-1, as well as histopathological examination and immunostaining for caspase-3, LC3, and Sirt-1 expression. Results: Administration of ADR caused significant elevation in liver enzymes (ALT, AST), lipid peroxidation (MDA), histopathological damage score and caspase-3 in liver tissues (p< 0.05) with a notable decrease in GSH, catalase, Nrf2, HO-1, LC3, and Sirt-1 genes expression (p< 0.05). Conversely, the application of intermittent fasting (IF) to ADR-treated rats caused significant attenuation of the raised liver enzymes (ALT, AST), lipid peroxidation (MDA), histopathological damage score and caspase-3 in liver tissues and significant improvement in the attenuated GSH, catalase, Nrf2, HO-1, Lc3 and Sirt-1 gene expression (p< 0.05). Conclusion: Intermittent fasting could potentially offer protection against ADR-induced hepatotoxicity in rats by reducing oxidative stress and apoptosis and modifying the expression of Nrf2/HO-1, LC3, and Sirt-1.</div> Adriamycin, Hepatoxicity, Intermittent Fasting, LC3, Nrf2, Oxidative Stress, Sirt-1. 235 249 http://rbmb.net/browse.php?a_code=A-10-1130-6&slc_lang=en&sid=1 Abdelaziz Hussein menhag@mans.edu.eg. 100319475328460022564 100319475328460022564 Yes Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt. Fathy Hamada Elsaid 100319475328460022565 100319475328460022565 No Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Assiut, Egypt. Wessam El-Sayed 100319475328460022566 100319475328460022566 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt. Elsayed Abdelfatah Eid 100319475328460022567 100319475328460022567 No Department of Internal Medicine, Faculty of Medicine, Delta University for Science and Technology, Mansoura (35516), Egypt. Omar Abd-Alhakem Ammar 100319475328460022568 100319475328460022568 No Department of Basic Sciences, Delta University for Science and Technology, Mansoura (35516), Egypt. Abdelnaser Badway 100319475328460022569 100319475328460022569 No Department of Medical Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia. Gamal Othman 100319475328460022570 100319475328460022570 No Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi Arabia. Shorouk Elsaeed Mohammed Elmorshdy 100319475328460022571 100319475328460022571 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt.