en بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;"><em><strong>Background:</strong></em> Chronic low-grade inflammation may be a cardinal pathophysiologic feature in the pathogenesis of frailty. Interferon-gamma (INF-&gamma;) is an understudied proinflammatory cytokine in frailty that induces many inflammatory pathways including the guanosine triphosphate cyclohydrolase 1 (GTP-CH1) pathway. Our aim was to evaluate the GTP-CH1 pathway in Egyptian frail elderly subjects.<br> <br> <em><strong>Methods:</strong></em> INF-&gamma;, neopterin, and nitric oxide (NO) levels were measured in 80 participants.<br> <br> <em><strong>Results:</strong></em> Both pre-frail and frail subjects had significantly higher levels of INF-&gamma;, neopterin and lower levels of NO than the control group. These biomarkers were associated with the risk of frailty with significant odds ratio.<br> <br> <strong><em>Conclusions: </em></strong>Elevated INF-&gamma; levels in frail subjects may activate the GTP-CH1 pathway. Elevated neopterin and reduced NO levels correlated with an active GTP-CH1 pathway. The risk of frailty increased with elevated INF-&gamma; and neopterin and decreased with elevated NO levels.</div> Frailty, GTP-CH1 pathway, INF-γ. 52 58 http://rbmb.net/browse.php?a_code=A-10-147-1&slc_lang=en&sid=1 Magda Mohamad magdaibrahim_bio@yahoo.com 10031947532846006372 10031947532846006372 Yes Medical Biochemistry Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Somaia Ebeid somaiaebeid@yahoo.com 10031947532846006373 10031947532846006373 No Geriatrics and Gerontology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Mohamed Shawky Khater drmkhater@yahoo.com 10031947532846006374 10031947532846006374 No Geriatrics and Gerontology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Mohamad Alsadany mohamadalsadany@yahoo.com 10031947532846006375 10031947532846006375 No Geriatrics and Gerontology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.