en ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder characterized by altered bowel habits and abdominal pain in the absence of a recognizable structural anomaly. The pathogenesis of IBS has been associated with inflammation and the expression of pro-inflammatory chemokines, such as CCL2 and CCL16. Our study aimed to investigate the relationship between the serum levels of CCL2 and CCL16 and IBS. Additionally, we examined how serum levels of these chemokines relate to IBS subtypes.<br> <br> <strong><em>Methods:</em></strong> Patients with IBS diagnosed according to the Rome III criteria participated in this study (n= 96). Healthy individuals with no history of allergic, autoimmune, chronic or active gastrointestinal infectious diseases were used as controls (n= 44). The serum levels of CCL2 and CCL16 was measured via enzyme-linked immunosorbent assay (ELISA).<br> <br> <strong><em>Results:</em></strong> A significant decrease in the serum levels of CCL16 and CCL2 was observed in the patients with IBS. Additionally, the serum levels of CCL16 in IBS patients with diarrhea (D-IBS) was significantly higher than those with the mixed IBS (M-IBS) subtype.<br> <br> <strong><em>Conclusions:</em></strong> The significant increase in the serum levels of CCL-16 in patients with D-IBS compared to patients with M-IBS suggests that CCL-16 may be used as an immunological biomarker to differentiate between these two subtypes.<br> &nbsp;</div> CCL-2, CCL-16, C-IBS, D-IBS, MCP-1, M-IBS, IBS. 9 14 http://rbmb.net/browse.php?a_code=A-10-48-4&slc_lang=en&sid=1 Mohammad Mahdi Hayatbakhsh m24672@yahoo.com 100319475328460017118 100319475328460017118 No Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology sciences, Kerman University of Medical Sciences, Kerman, Iran. Arezoo Gowhari Shabgah gohariarezoo@yahoo.com 100319475328460017119 100319475328460017119 No Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Saeed Pishgouyi dr_s_p_md@yahoo.com 100319475328460017120 100319475328460017120 No Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. Jalil Tavakiol Afshari tavakolaj@mums.ac.ir 100319475328460017121 100319475328460017121 No Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Hadi Zeidabadi hadizeydabadi@gmail.com 100319475328460017122 100319475328460017122 No Department of Gastroenterology, Afzalipour hospital, Kerman University of Medical Sciences, Kerman, Iran. Mojgan Mohammadi mohammadimzh@mums.ac.ir 100319475328460017123 100319475328460017123 Yes Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.