en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background: </em></strong><em>Staphylococcus aureus</em> is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals.<br> <strong><em>Methods:</em></strong> The polysaccharide intercellular adhesin (PIA) and <em>S. epidermidis</em> rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host.<br> <strong><em>Results:</em></strong> Purification of the macromolecules was confirmed by FTIR and Western blotting.<br> <strong><em>Conclusions:</em></strong> The <em>S. epidermidis</em> SesC protein and PIA were successfully purified. Both are considered as vaccine candidates.</div> Biofilm formation, Polysaccharide Intracellular Adhesin, Staphylococcus aureus. 161 167 http://rbmb.net/browse.php?a_code=A-10-137-2&slc_lang=en&sid=1 Sara Bahonar Sarah.bahonar@gmail.com 100319475328460017267 100319475328460017267 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. & Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran. Maryam Ghazvinian maryam.ghazvinian34@gmail.com 100319475328460017268 100319475328460017268 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. Mohamad Reza Haghshenas haghshenas2001@yahoo.com 100319475328460017269 100319475328460017269 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. Hamid Reza Goli Goli59@gmail.com 100319475328460017270 100319475328460017270 No Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. Bahman Mirzaei b.mirzaei@mazums.ac.ir 100319475328460017271 100319475328460017271 Yes Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. & Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.