Reports of Biochemistry and Molecular Biology

en Swapping of The N-Terminal Domain of Human Topoisomerase 1B with the Corresponding Plasmodium Falciparum Counterpart Strongly Impairs Enzyme Activity زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: DNA topoisomerases 1B are a class of ubiquitous enzyme that solves the topological problems associated with biological processes such as replication, transcription and recombination. Numerous sequence alignment of topoisomerase 1B from different species shows that the lengths of different domains as well as their amino acids sequences are quite different. In the present study a hybrid enzyme, generated by swapping the N-terminal of Plasmodium falciparum into the corresponding domain of the human, has been characterized. Methods: The chimeric enzyme was generated using different sets of PCR. The in vitro characterization was carried out using different DNA substrate including radio-labelled oligonucleotides. Results: The chimeric enzyme displayed slower relaxation activity, cleavage and re-ligation kinetics strongly perturbed when compared to the human enzyme. Conclusions: These results indicate that the N-terminal domain has a crucial role in modulating topoisomerase activity in different species.  </div> N-terminal domain, Plasmodium falciparum topoisomerase 1B, Topoisomerase 1B. 366 375 http://rbmb.net/browse.php?a_code=A-10-286-3&slc_lang=en&sid=1 Jagadish Babu Dasari jagadishdrdasari@gmail.com 100319475328460017406 100319475328460017406 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy. Bini Chhetri Soren binichhetri.14@gmail.com 100319475328460017407 100319475328460017407 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy. Alessio Ottaviani ottaviani.alessio25@gmail.com 100319475328460017408 100319475328460017408 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy. Cinzia Tesauro cinzia.tesauro@gmail.com 100319475328460017409 100319475328460017409 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy & Present address: Department of Molecular Biology and Genetics, University of Aarhus, C.F MøllersAllè 3, 8000 Aarhus C, Denmark. Simona Marino simona_992@hotmail.it 100319475328460017410 100319475328460017410 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy. Beatrice Messina beatrice.messina702@gmail.com 100319475328460017411 100319475328460017411 No Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy. Paola Fiorani paola.fiorani@uniroma2.it 100319475328460017412 100319475328460017412 Yes Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy & Institute of Translational Pharmacology, National Research Council, CNR, Via Del Fosso del Cavaliere 100, Rome 00133, Italy.