en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Glutathione S-transferases (GSTs) protect cells from oxidative stress (OS). In humans, the GST omega class contains two expressed genes, <em>GSTO1</em> and <em>GSTO2</em>. Because OS is involved in the pathogenesis of polycystic ovary syndrome (PCOS), the aim of this study was to investigate the relationship between <em>GSTO1</em> A140D (rs4925) and <em>GSTO2</em> N142D (rs156697) polymorphisms in PCOS patients.<br> <br> <strong><em>Methods:</em></strong> 175 PCOS patients and 161 healthy controls were selected among women in Kermanshah province, Iran. <em>GSTO1</em> and <em>GSTO2</em> were genotyped using allele-specific PCR (AS-PCR) and PCR-RFLP, respectively.<br> <br> <strong><em>Results:</em></strong> For <em>GSTO1</em>, the DD genotype and the D allele led to 2.17- (<em>P</em>= 0.02) and 1.5-fold (<em>P</em>= 0.01) increases, respectively, in the odds ratios for PCOS. No significant difference was found between control and patient groups for the <em>GSTO2</em> N142D genotype or allele frequency. <em>GSTO1</em> and <em>GSTO2</em> genotype interaction analysis showed that individuals with the <em>GSTO1</em> AD or DD genotypes and the <em>GSTO2 </em>NN or DN genotypes had a 1.53-fold (<em>P</em>= 0.007) increase in PCOS risk over <em>GSTO1</em> AA and <em>GSTO2</em> DD individuals.<br> <br> <strong><em>Conclusions:</em></strong> The <em>GSTO1</em> A140D polymorphism is a risk factor for PCOS.</div> Gene polymorphism, GSTO1, GSTO2, Oxidative stress, PCOS. 8 13 http://rbmb.net/browse.php?a_code=A-10-389-1&slc_lang=en&sid=1 Seyyed Shahram Miraghaee shahram.miraghaee@yahoo.com 100319475328460017477 100319475328460017477 No Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Maryam Sohrabi mery_sohrabi@yahoo.com 100319475328460017478 100319475328460017478 No Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Cyrus Jalili cjalili@yahoo.com 100319475328460017479 100319475328460017479 No Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Fariborz Bahrehmand FariborzBahrehmand@gmail.com 100319475328460017480 100319475328460017480 Yes Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.