en زیست شناسی سلولی Cell Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> The gold standard for detection of bladder cancer is cystoscopy, which is an invasive and complicated procedure. Our study was conducted to find a tumor marker with high specificity, sensitivity, and accuracy for the diagnosis of bladder cancer.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Serum samples were collected from 58 bladder cancer patients and 60 healthy control subjects. Levels of lipid-bound sialic acid (LBSA), and protein-bound sialic acid (PBSA) were measured spectrophotometrically by Aminoff&rsquo;s method.</p> <p style="text-align: justify;"><strong>Results:</strong> Mean levels of both markers were found to be significantly higher in the patients than the healthy controls. Positive correlations were observed between serum levels of lipid- (r=0.283, p<0.05) and protein- bound (r=0.56, p<0.05) sialic acids and the grade of malignancy. To differentiate patients with bladder tumors from healthy controls, cut-offpoints were determined for each of the two parameters based on Receiver Operating Characteristic (ROC) curve analysis (LBSA=21.25 mg/dL, PBSA=6.15 mg/dL). The data showed good sensitivities (LBSA=89%, PBSA=79%), specificities (LBSA=70%, PBSA=70%) and accuracies (LBSA=83%, PBSA=81%) for both markers.</p> <p style="text-align: justify;"><em><strong>Conclusion:</strong></em> Measuring serum LBSA and PBSA by this simple, reproducible, noninvasive, and inexpensive method can accurately discriminate cancer patients from healthy individuals.</p> N-Acetylneuraminic Acid, Tumor Markers, Urinary Bladder Neoplasms 70 75 http://rbmb.net/browse.php?a_code=A-10-1-24&slc_lang=en&sid=1 Shima Habibi 10031947532846005979 10031947532846005979 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Hassan Jamshidian 10031947532846005980 10031947532846005980 No Department of Urology, Medical School, Tehran University of Medical Sciences, Tehran, Iran. Mahdi Kadivar 10031947532846005981 10031947532846005981 No Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran. Mohammad Reza Eshraghian 10031947532846005982 10031947532846005982 No Department of Epidemiology & Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hassan Javanbakht 10031947532846005983 10031947532846005983 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Hoda Derakhshanian 10031947532846005984 10031947532846005984 No Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mahmoud Djalali mjalali87@yahoo.com 10031947532846005985 10031947532846005985 Yes Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran