en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Prostate cancer (PC) is one of the most abundant cancers among men, and In Iran, has been responsible for 6% of all deaths from cancer in men.<em> NUF2</em> and <em>GMNN</em> genes are considered as loci of susceptibility to tumorigenesis in humans. Alterations in expression of these genes have been reported in various malignancies. The aim of our study was to test whether different <em>NUF2</em> and <em>GMNN</em> expression levels are associated with PC incidence and hence, might be considered as new molecular tools for PC screening.<br> <br> <strong><em>Methods:</em></strong> Biopsy samples from 40 PC patients and 41 healthy Iranian men were used to determine the relative gene expression. After RNA extraction and cDNA synthesis, samples were analyzed using TaqMan Quantitative Real time PCR. Patients&rsquo; background information, included smoking habits and family histories of PC, were recorded. Stages and grades of their PC were classified by the TNM tumor, node, metastasis (TMN) staging system based on standard guidelines.<br> <br> <strong><em>Results:</em></strong> <em>NUF2</em> expression did not significantly differ between the groups, while <em>GMNN</em> expression was significantly greater in the PC specimens than in the controls.<br> <br> <strong><em>Conclusions:</em></strong> Regarding the significant role of<em> GMNN</em> in various tumor phenotypes, and its importance in PC progression, the alteration in <em>GMNN </em>expression in PC samples vs. controls indicate that the genetic profiling of this cancer might be considered to personalize therapy for each patient in the future.</div> Family history, Geminin (GMNN), Tumor staging, NUF2, Prostate cancer. 224 232 http://rbmb.net/browse.php?a_code=A-10-576-3&slc_lang=en&sid=1 Shaghayegh Pezeshki 100319475328460012631 100319475328460012631 No Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Payam Hashemi 100319475328460012632 100319475328460012632 No School of Medicine, Tehran University of Medical Science, Tehran, Iran. Alireza Salimi Alireza1salimi@gmail.com 100319475328460012633 100319475328460012633 No Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Sheida Ebrahimi 100319475328460012634 100319475328460012634 No Department of Genetic, Faculty of biology, Yazd University, Yazd, Iran Mandana Javanzad 100319475328460012635 100319475328460012635 No Medical Branch, Islamic Azad University, Tehran, Iran. Amir Monfaredan amir.monfaredan@yahoo.com 100319475328460012636 100319475328460012636 Yes Department of Hematology, Faculty of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.