fa زیست شناسی سلولی Cell Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> Marine natural products contain a wide range of bioactive compounds with therapeutic properties that have revealed crucial properties in the treatment of some diseases. Some of these compounds have recently received considerable attention for drug discovery. In this study we examined the anti-angiogenic effect of saponin isolated from <em>Holothuria leucospilota</em> (sea cucumber) through evaluation of vascular endothelial growth factor D (VEGF-D) and transforming growth factor-&beta; (TGF&beta;) expression in a breast cancer cell line.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> To investigate the effect of SCS on VEGF-D and TGF-&beta; expression in breast cancer cells, the cells were treated with various concentrations of sample. After 48 h the viability of the cells was evaluated by trypan blue staining, and VEGF-D and TGF&beta; mRNA expression was were evaluated by real time-PCR.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> Our results revealed that SCS can suppress cell viability and VEGF-D and TGF&beta; mRNA expression in breast cancer cells. Sea cucumber saponin at a concentration of 12 &mu;g/ml inhibited VEGF-D and TGF&beta; expression more than 90% compared with controls.</p> <p style="text-align: justify;"><em><strong>Conclusion: </strong></em>Findings suggest that SCS could inhibit tumor growth via inhibition of angiogenesis.</p> Angiogenesis, Anticancer, Saponin, Sea cucumber 25 31 http://rbmb.net/browse.php?a_code=A-10-1-47&slc_lang=fa&sid=1 Mozhgan Soltani 10031947532846006092 10031947532846006092 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Kazem Parivar 10031947532846006093 10031947532846006093 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Javad Baharara baharara@yahoo.com 10031947532846006094 10031947532846006094 Yes Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Mohammad Amin Kerachian 10031947532846006095 10031947532846006095 No Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Javad Asili 10031947532846006096 10031947532846006096 No Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran