en زیست شناسی ملکولی Molecular Biology <p style="text-align: justify;"><em><strong>Background:</strong></em> Half of the cases of vision loss in people under 60 years of age have been attributed to age-related macular degeneration (AMD). This is a multifactorial disease with late onset. It has been demonstrated that many different genetic loci are implicated in the risk of developing AMD in different populations. In the current study, we investigated the association of high-temperature &lrm;requirement A-1 (HTRA1) gene polymorphisms with the risk of developing AMD in the Iranian population.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Genomic DNA samples were extracted from 120 patients with AMD and 120 healthy age- and sex-matched controls. A 385 base-pair fragment of the HTRA1 gene promoter region was amplified using the polymerase chain reaction (PCR) technique and sequenced. The frequencies of the alleles were calculated and statistical analysis was performed using SPSS software.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> Our study demonstrated that the rate of polymorphisms rs11200638 -625 G>A and rs2672598 -487T>C were significantly greater in AMD patients than in healthy controls from the Iranian population.</p> <p style="text-align: justify;"><em><strong>Conclusions:</strong></em> The results of our study indicate that HTRA1 gene promoter region polymorphisms are associated with the risk of developing AMD in the Iranian population.</p> HTRA1, Iran, Macular Degeneration, Single Nucleotide Polymorphisms 43 49 http://rbmb.net/browse.php?a_code=A-10-1-49&slc_lang=en&sid=1 Mohammad Askari 10031947532846006104 10031947532846006104 No Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran Amin Reza Nikpoor 10031947532846006105 10031947532846006105 No Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Fazel Gorjipour 10031947532846006106 10031947532846006106 No Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohsen Mazidi 10031947532846006107 10031947532846006107 No Biochemistry and Nutrition Research Center, Mashhad University of Medical Sciences, Mashhad, Mohammad Hosein Sanati 10031947532846006108 10031947532846006108 No National Institute for Genetic Engineering and Biotechnology, Tehran, Iran Hajar Aryan 10031947532846006109 10031947532846006109 No Fazeli-Sanati Genetic Laboratory, Tehran, Iran Alireza Irani 10031947532846006110 10031947532846006110 No Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Khalil Ghasemi Falavarjani 10031947532846006111 10031947532846006111 No Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Hossein Nazari 10031947532846006112 10031947532846006112 No Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Kazem Mousavizadeh mousavik@gmail.com 10031947532846006113 10031947532846006113 Yes Cellular and Molecular Research Center, Laboratory of Basic Sciences, Iran University of Medical Sciences, Tehran, Iran