en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Epithelial malignancy in lung cancer, which is initiated with myofibroblast differentiation and remodeling, promotes hypoxia and intracellular ROS generation most affected by the prototypical enzyme, NADPH oxidase 4 (<em>NOX4</em>). In addition, nuclear factor erythroid 2-related factor 2 (<em>Nrf2</em>) acts as a critical transcription factor by stimulating antioxidant proteins as redox homeostasis regulators. The aim of this study was to investigate a possible correlation between lung tissue <em>NOX4</em> and <em>Nrf2</em> genes (<em>NOX4</em> and <em>Nrf2</em>) mRNA expression and bronchoalveolar lavage fluid (BALF) protein expression in non-small-cell lung carcinoma (NSCLC) patients.<br> <br> <strong><em>Methods:</em></strong> Samples from 25 patients with various NSCLC types and stages and 20 healthy controls were collected. <em>NOX4</em> and <em>Nrf2</em> mRNA were measured by qRT-PCR, and protein by western blot analysis.<br> <br> <strong><em>Results:</em></strong> NOX4 mRNA and protein expression was significantly up-regulated in NSCLC patients&rsquo; lung tissues and BALFs (p= 0.03 and 0.01, respectively). In addition, by adjusting for age, sex, and NSCLC types and stages, a significant and positive correlation was observed between <em>NOX4</em> and <em>Nrf2</em> mRNA expression (r= 0.927, p= 0.001). <a name="_Hlk77142324">This was also true when not adjusted as above (r= 0.944, p< 0.001</a>).<br> <br> <strong><em>Conclusions:</em></strong> <em>NOX4</em> mRNA and protein expression is significantly up-regulated in NSCLC patients&rsquo; lung tissues and BALFs, and <em>NOX4</em> and <em>Nrf2 </em>mRNA expression is positively correlated in NSCLC tissues.<br> &nbsp;</div> Gene expression, Non-small-cell lung cancer, NOX4, Nrf2. 327 333 http://rbmb.net/browse.php?a_code=A-10-770-1&slc_lang=en&sid=1 Nasim Bakhtiyari 100319475328460012687 100319475328460012687 Yes Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Akbar Sharifi 100319475328460012688 100319475328460012688 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Younes Aftabi 100319475328460012689 100319475328460012689 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Neda Gilani 100319475328460012690 100319475328460012690 No Department of Statistics and Epidemiology, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran. Venus Zafari 100319475328460012691 100319475328460012691 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Venus Zafari 100319475328460012692 100319475328460012692 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Atefeh Ansarin 100319475328460012693 100319475328460012693 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Ensiyeh Seyedrezazadeh e.s.rezazadeh@tbzmed.ac.ir. 100319475328460012694 100319475328460012694 No Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.