en بیوشیمی Biochemistry <p style="text-align: justify;"><em><strong>Background: </strong>Neisseria meningitidis</em>, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5&ndash;15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein antigen (ACP-MIP-PilQ) from <em>N. meningitidis serogroup</em> B strain.</p> <p style="text-align: justify;"><em><strong>Methods: </strong></em>Biochemical properties and multiple alignments were predicted by appropriate web servers. Secondary molecular structures were predicted based on Chou and Fasman, Garnier-Osguthorpe-Robson, and Neural Network methods. Tertiary modeling elucidated conformational properties of the chimeric protein. Proteasome cleavage and transporter associated with antigen processing (TAP) binding sites, and T- and B-cell antigenic epitopes, were predicted using bioinformatic web servers.</p> <p style="text-align: justify;"><em><strong>Results: </strong></em>Based on our <em>in silico</em> and immunoinformatics analyses, the ACP-MIP-PilQ protein (AMP) can induce high-level cross-strain bactericidal activity. In addition, several immune proteasomal cleavage sites were detected. The 22 epitopes associated with MHC class I and class II (DR) alleles were confirmed in the AMP. Thirty linear B-cell epitopes as antigenic regions were predicted from the full-length protein.</p> <p style="text-align: justify;"><em><strong>Conclusion: </strong></em>All predicted properties of the AMP indicate it could be a good candidate for further immunological <em>in vitro</em> and<em> in vivo</em> studies.</p> Chimeric protein, In silico, Neisseria meningitides, Serogroup B, Vaccine 50 59 http://rbmb.net/browse.php?a_code=A-10-1-50&slc_lang=en&sid=1 Mehrdad Gholami 10031947532846006114 10031947532846006114 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Alireza Salimi Chirani Alireza.salimichirani@gmail.com 10031947532846006115 10031947532846006115 Yes Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mona Moshiri 10031947532846006116 10031947532846006116 No Department of Pathobiology, Division of Microbiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mansour Sedighi 10031947532846006117 10031947532846006117 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Abazar Pournajaf 10031947532846006118 10031947532846006118 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Masoud Tohidfar 10031947532846006119 10031947532846006119 No Agricultural Biotechnology Research Institute of Iran, Tehran, Iran Gholamreza Irajian 10031947532846006120 10031947532846006120 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran