en میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Antibiotics called macrolide, lincosamide and streptogramin B (MLSB) are being used to treat&nbsp;staphylococci infections. Multiple pathways that impart resistance to MLSB antibiotics have been confirmed&nbsp;to cause clinical failure. The present work aimed to determine the frequency of constitutive and inducible&nbsp;clindamycin resistant among coagulase-negative staphylococci (CoNS) isolates of different clinical samples&nbsp;in Al-Basrah governorate, Iraq.<br> <br> <em><strong>Methods:</strong></em> The 28 CoNS, traditional techniques and the Vitek&reg;2 system were used to identify the isolates.&nbsp;The disk diffusion technique was used to detect methicillin resistance and antibiotic sensitivity patterns via&nbsp;cefoxitin, gentamicin, ciprofloxacin, amikacin, teicoplanin, linezolid, doxycycline and vancomycin disks.&nbsp;Erythromycin and clindamycin antibiotic disks was used to detect the inducible and constitutive&nbsp;clindamycin resistance as well as a D-test according to CLSI guidelines.<br> <br> <strong><em>Results: </em></strong>Among 28 CoNS isolated, the Staphylococcus aureus 11(39.29%), Staphylococcus epidermidis&nbsp;7(25 %), Staphylococcus haemolyticus 4(14.29%) and Staphylococcus saprophyticus 3 (10.71%) were&nbsp;predominant isolated species. Out of 28 CoNS isolates, 15(53.57%) were methicillin resistant coagulasenegative&nbsp;staphylococci (MRCoNS) isolates and 13(46.43%) were methicillin sensitive coagulase-negative&nbsp;staphylococci (MSCoNS) isolates. The 15(53.57%) isolates out of 28 CoNS, showed erythromycin&nbsp;resistance while 6(40%) isolates out of 15 CoNS, showed inducible macrolide-lincosamide-streptogramin&nbsp;B (iMLSB) and 2(13.3%) of CONS isolated showed constitutive macrolide-lincosamide-streptogramin B&nbsp;(cMLSB).<br> <br> <em><strong>Conclusions:</strong></em> In order to achive the best result in choosing the suitable treatment and avoiding the loses the&nbsp;money and time, it is better to use the D-test for inducible clindamycin resistance in the daily routine work&nbsp;of antibiotic susceptibility testing in hospital and private clinical laboratories.</div> Anti-Bacterial Agents, Clindamycin, Staphylococcus 30 35 http://rbmb.net/browse.php?a_code=A-10-864-1&slc_lang=en&sid=1 Saad Shakir Mahdi Al-Amara ha3848853@gmail.com 100319475328460012317 100319475328460012317 Yes Department of Biology, College of Science, University of Basrah, Iraq.