en ایمنی شناسی Immunology <p style="text-align: justify;"><em><strong>Background:</strong></em> <em>Platanus </em>species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or 3 allergen in <em>P. orientalis</em> pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Pollen samples were collected from three polluted and two unpolluted sites in Mashhad, northeast Iran. Recombinant Pla or 3 was expressed and used for polyclonal antibody production in rabbit. A sandwich ELISA was developed and validated to quantify Pla or 3 levels in pollen extracts from the different sites.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> The coefficients of variation (CVs) for the intra- and inter- day assays were less than 5 and 18%, respectively. The working range of the standard curve was between 0.1 and 25 ng/ml, with the detection limit being 0.037 ng/ml. The recovery percentage was 88-106.4% at working concentrations from 0.31 to 26.5 ng/ml. Pla or 3 levels were significantly greater in pollens grown near high-traffic roads than in those grown in rural regions (p < 0.0001).</p> <p style="text-align: justify;"><em><strong>Conclusions: </strong></em>A sandwich ELISA was developed and validated to quantify Pla or 3 in pollen extracts. Using this validated ELISA, we showed a substantial difference between the amounts of Pla or 3 in pollens grown in different environments. This finding should be considered in developing public policies to reduce traffic pollution, which leads to reduced allergic reactions in atopic subjects.</p> Pathogenesis-related proteins, Pla or 3, Platanus orientalis, Sandwich ELISA 40 45 http://rbmb.net/browse.php?a_code=A-10-1-63&slc_lang=en&sid=1 Farnaz Sedghy 10031947532846006172 10031947532846006172 No Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran Mojtaba Sankian 10031947532846006173 10031947532846006173 No Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran Maliheh Moghadam 10031947532846006174 10031947532846006174 No Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran Abdol-Reza Varasteh varasteha@mums.ac.ir 10031947532846006175 10031947532846006175 Yes Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran