en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> Prostate cancer is known as one of the most prevalent health disorders in the male population&nbsp;globally. The aim of the current study was to evaluate the effects of separate and concomitant use of MK-2206 and salinomycin on prostate cancer cell line.<br> <br> <strong><em>&nbsp;Methods:</em></strong> The antitumor potential of separate and concomitant use of MK-2206 and salinomycin was&nbsp;evaluated in a panel of prostate cancer cell line (PC-3). To get insights into the underlying mechanism of&nbsp;action, different assays including the rate of apoptosis, cell viability, and gene expression were performed&nbsp;in treated prostate cancer cells.<br> <br> <strong><em>Results:</em></strong> A significant reduction was detected in the viability percentage of prostate cancer cells (p< 0.001)&nbsp;and the rate of Akt expression (p< 0.001) in all salinomycin, MK-2206, and salinomycin+MK-2206 groups&nbsp;compared to the negative control group. Furthermore, in comparison with the negative control group, there&nbsp;was a notable increase in both the rate of Bad expression (p< 0.001) and prostate cancer cells apoptosis after&nbsp;salinomycin, MK-2206, and salinomycin+MK-2206 treatments. Moreover, the concomitant use of&nbsp;salinomycin+MK-2206 revealed synergistic improvements regarding the viability of prostate cancer cells&nbsp;and the rate of the Akt and Bad expressions compared to the separate administration of salinomycin and&nbsp;MK-2206 (all p< 0.05).<br> <br> <em><strong>Conclusions:</strong></em> The findings of the present study may contribute to improving the efficacy of the therapies&nbsp;regarding the management of prostate cancer and providing a beneficial strategy in clinical trials.</div> Apoptosis, Gene Expression, MK 2206, Prostatic Neoplasms, Salinomycin. 157 165 http://rbmb.net/browse.php?a_code=A-10-907-1&slc_lang=en&sid=1 Mohamadreza Savaee 100319475328460012372 100319475328460012372 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Ali Bakhshi 100319475328460012373 100319475328460012373 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Fatemeh Yaghoubi 100319475328460012374 100319475328460012374 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Fatemeh Pourrajab mina_poorrajab@yahoo.com. 100319475328460012375 100319475328460012375 Yes Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Koorosh Goodarzvand Chegini Kooroshgoodarzvandchegini@gmail.com 100319475328460012376 100319475328460012376 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.